Plasmon-Enhanced Colorimetric ELISA with Single Molecule Sensitivity
Plasmon-Enhanced Colorimetric ELISA with Single Molecule Sensitivity
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DOI：
10.1021/nl2006092
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发表时间：
2011-04-01
期刊：
影响因子：
10.8
通讯作者：
Kall, Mikael
中科院分区：
文献类型：
--
作者：
Chen, Si;Svedendahl, Mikael;Kall, Mikael
鲁棒但超灵敏的生物传感器具有检测低丰度生物标志物的能力，可以彻底改变临床诊断，使癌症，神经系统疾病和感染的早期检测成为可能。我们利用局部表面等离子体共振（LSPR）折射率传感和众所周知的酶联免疫吸附测定相结合，开发了一种简单的单分子灵敏度比色生物传感方法。该技术是基于对大量分离的金纳米颗粒的光谱成像。每个颗粒结合可变数量的辣根过氧化物酶（HRP）酶分子，在颗粒表面催化局部沉淀反应。酶促反应极大地放大了LSPR散射最大值λ (max)的位移，使得检测每个颗粒中只有一个或几个HRP分子的存在成为可能。
Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, lambda(max), and makes it possible to detect the presence of only one or a few HRP molecules per particle.