An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture
An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture
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DOI：
10.1002/jbm.a.30870
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Yung, Lin Yue Lanry
中科院分区：
文献类型：
--
作者：
Zhong, Shaoping;Teo, Wee Eong;Yung, Lin Yue Lanry
纳米纤维支架具有明确的结构，模拟天然细胞外基质模拟物，在许多特定的组织工程和器官再生应用中具有重要的潜力。本研究描述了静电纺丝法制备排列的胶原纳米纤维支架。将这些支架的结构和体外性能与随机胶原支架进行比较。所有胶原蛋白支架首先在戊二醛蒸气中交联，以提高生物稳定性并保持初始纳米尺度的完整性。从兔结膜成纤维细胞体外培养结果来看，与随机支架相比，定向支架由于纤维定向排列，具有较低的细胞粘附性，但具有较高的细胞增殖能力。纤维的排列可以控制细胞的方向，并在纤维的纵向上加强细胞体与纤维的相互作用。(c) 2006 Wiley期刊公司
Fabrication of nanofibrous scaffolds with well-defined architecture mimicking native extracellular matrix analog has significant potentials for many specific tissue engineering and organs regeneration applications. The fabrication of aligned collagen nanofibrous scaffolds by electrospinning was described in this study. The structure and in vitro properties of these scaffolds were compared with a random collagen scaffold. All the collagen scaffolds were first crosslinked in glutaraldehyde vapor to enhance the biostability and keep the initial nano-scale dimension intact. From in vitro culture of rabbit conjunctiva fibroblast, the aligned scaffold exhibited lower cell adhesion but higher cell proliferation because of the aligned orientation of fibers when compared with the random scaffold. And the alignment of the fibers may control cell orientation and strengthen the interaction between the cell body and the fibers in the longitudinal direction of the fibers. (c) 2006 Wiley Periodicals, Inc.