Fretting about FRET: correlation between kappa and R.
Fretting about FRET: correlation between kappa and R.
复制标题
对 FRET 的烦恼：kappa 和 R 之间的相关性。
作者：
Darren B. VanBeek;M. Zwier;J. M. Shorb;B. Krueger
采用分子动力学模拟方法研究了两种荧光探针与典型蛋白质鸡蛋清溶菌酶（HEWL）的结构动力学。供体探针（D）通过琥珀酰亚胺基团连接，与常用的马来酰亚胺缀合化学一致，受体探针（A）结合到蛋白质中，如HEWL和染料曙红Y天然发生的那样。发现偏离理论值显着和高度的相关性之间的取向因子kappa和距离R观察。使用几种可能的固定A取向来量化相关性，并且发现kappa和R之间的相关性高达0.80，kappa（2）和R之间的相关性高达0.68。这种相关性的存在突出了这样一个事实，即基本上所有的荧光检测共振能量转移研究都假设κ和R是独立的-在这里研究的系统中，这一假设显然是不合理的。相关性导致量和< R（-）（δ）>相差1.6倍。观察到的κ和R之间的相关性是由D和HEWL之间的琥珀酰亚胺键引起的，发现其相对不灵活。
Molecular dynamics simulations were used to examine the structural dynamics of two fluorescent probes attached to a typical protein, hen egg-white lysozyme (HEWL). The donor probe (D) was attached via a succinimide group, consistent with the commonly-used maleimide conjugation chemistry, and the acceptor probe (A) was bound into the protein as occurs naturally for HEWL and the dye Eosin Y. The is found to deviate significantly from the theoretical value and high correlation between the orientation factor kappa and the distance R is observed. The correlation is quantified using several possible fixed A orientations and correlation as high as 0.80 is found between kappa and R and as high as 0.68 between kappa(2) and R. The presence of this correlation highlights the fact that essentially all fluorescence-detected resonance energy transfer studies have assumed that kappa and R are independent--an assumption that is clearly not justified in the system studied here. The correlation results in the quantities and < R(-)(6)> differing by a factor of 1.6. The observed correlation between kappa and R is caused by the succinimide linkage between the D and HEWL, which is found to be relatively inflexible.
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影响因子：
2.9
作者：
Dagmar Klostermeier;David P. Millar
通讯作者：
Dagmar Klostermeier;David P. Millar
钙泵存在下受磷蛋白的解聚：荧光能量转移研究。
影响因子：
2.9
作者：
Reddy,LG;Jones,LR;Thomas,DD
通讯作者：
Thomas,DD
DOI：
10.1073/pnas.0408164102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Schuler, B;Lipman, EA;Eaton, WA
通讯作者：
Eaton, WA
影响因子：
15
作者：
Adams, SR;Campbell, RE;Tsien, RY
通讯作者：
Tsien, RY