Cell proliferation and apoptosis in the anterior pituitary of chicken during inhibition and resumption of laying.
Cell proliferation and apoptosis in the anterior pituitary of chicken during inhibition and resumption of laying.
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DOI：
10.1006/gcen.2001.7739
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发表时间：
2002
影响因子：
2.7
通讯作者：
V. S. Chowdhury;Y. Yoshimura
中科院分区：
文献类型：
--
作者：
V. S. Chowdhury;Y. Yoshimura
本研究的目的是确定是否组织再生的垂体前叶细胞增殖和凋亡发生在抑制和恢复产卵。白色来航产蛋鸡通过停饲抑制产蛋。在停止产蛋的第四天恢复喂食。所有鸡在组织收集前1 h腹腔注射溴脱氧尿苷（BrdU）。从以下组的母鸡中收集垂体前叶：预处理（PT）、饥饿后3天和5天（分别为3DS和5DS）、停止产蛋后3天（3DC）、停止产蛋后10天（10DC，恢复饲喂后6天）、恢复产蛋当天和恢复产蛋后1周（分别为RL和1WRL）。通过BrdU免疫染色和末端脱氧核苷酸转移酶介导的生物素化脱氧尿苷三磷酸缺口末端标记（TUNEL）检测增殖细胞和凋亡细胞。还进行了垂体前叶激素的免疫染色。头叶BrdU阳性细胞在RL中的频率高于PT、3DC和1WRL。尾叶BrdU阳性细胞频率RL大于PT。RL组头、尾叶TUNEL阳性细胞明显增多。它们在头叶的频率在RL中比在PT中更高，并且在RL的尾叶中比在任何其他组的鸟中更高。10DC和RL中FSH样细胞的面积大于PT至3DC，RL中LH样细胞的面积大于3DS至3DC。PRL样细胞减少至3DC，然后逐渐增加至1WRL。生长激素，促甲状腺激素，促肾上腺皮质激素样细胞面积呈增加的趋势，直到3DC，与减少之后。10DC至1WRL的FSH样细胞和RL的LH样细胞的大小均大于停产前后。这些结果表明，在抑制和恢复产蛋细胞增殖和凋亡发生在垂体前叶组织，这可能会导致组织的年轻化，以改善这一器官的功能。
The goal of this study was to determine whether tissue rejuvenation of the anterior pituitary with cell proliferation and apoptosis occurs during inhibition and resumption of egg-laying. White Leghorn laying hens were subjected to inhibition of laying by feed withdrawal. Feeding was resumed on the fourth day of egg-laying cessation. All birds were injected ip with bromodeoxyuridine (BrdU) 1 h before tissue collection. The anterior pituitary glands were collected from hens of the following groups: pretreatment (PT), 3 and 5 days after starvation (3DS and 5DS, respectively), 3 days after cessation of laying (3DC), 10 days after cessation of laying (10DC, 6 days after resumption of feeding), and the day of and 1 week after resumption of laying (RL and 1WRL, respectively). They were processed for the detection of proliferating cells and apoptotic cells by BrdU immunostaining and terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick-end-labeling (TUNEL). Immunostaining for the anterior pituitary hormones was also conducted. In the cephalic lobe the BrdU-positive cells showed a higher frequency in RL than in PT, 3DC, and 1WRL. BrdU-positive cell frequency in the caudal lobe was greater in RL than in PT. TUNEL-positive cells in both cephalic and caudal lobes were increased markedly in the RL group. Their frequency in the cephalic lobe was greater in RL than in PT, and that in the caudal lobe of RL was higher than in any other group of birds. The areas of FSH-like cells in 10DC and RL were greater than those in PT to 3DC, and those of LH-like cells in RL were greater than those of 3DS to 3DC. PRL-like cells were decreased until 3DC and then gradually increased until 1WRL. GH-, TSH-, and ACTH-like cell areas showed tendencies to increase until 3DC, with decreasing thereafter. The sizes of FSH-like cells in 10DC to 1WRL and LH-like cells in RL were larger than those around cessation of laying. These results suggest that during inhibition and resumption of laying cell proliferation and apoptosis occur in the anterior pituitary tissue, which may cause a rejuvenation of tissue to improve the function of this organ.