The Submergence Tolerance Regulator SUB1A Mediates Crosstalk between Submergence and Drought Tolerance in Rice
The Submergence Tolerance Regulator SUB1A Mediates Crosstalk between Submergence and Drought Tolerance in Rice
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DOI：
10.1105/tpc.110.080325
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发表时间：
2011-01-01
期刊：
影响因子：
11.6
通讯作者：
Bailey-Serres, Julia
中科院分区：
文献类型：
--
作者：
Fukao, Takeshi;Yeung, Elaine;Bailey-Serres, Julia
淹水和干旱是雨水灌溉农田水稻生产的主要制约因素，在一个作物周期内，这两个因素可能会相继发生。SuB1a是一种在有限的水稻材料中发现的ERF转录因子，它在淹水过程中抑制乙烯的产生和赤霉酸的响应，节省碳水化合物储备，并显著延长耐力。在这里，我们评估了SUB1A在适应脱水中的功能作用。对有无SUB1a基因的比较分析表明，在营养生长期，SUB1a通过减少叶片失水和膜脂过氧化作用，增加适应脱水的相关基因的表达，促进了干旱的恢复。SUB1A的过表达增强了ABA的反应性，从而激活了胁迫诱导的基因表达。矛盾的是，即使土壤有足够的水分，营养组织在脱水时也会经历脱水，这表明叶片干燥发生在洪水事件的自然发展过程中。去潜水导致与适应脱水相关的基因转录上调，其中SUB1A型的诱导作用更高。此外，SUB1A还抑制了干旱和淹水期间气生组织中活性氧物种(ROS)的积累。一直以来，SuB1A增加了编码ROS清除酶的转录本的丰度，从而增强了对氧化应激的耐受性。因此，除了提供强大的耐淹水能力外，SUB1A还可以提高在干旱期间脱水后的快速脱水和水分亏缺的存活率。
Submergence and drought are major constraints to rice (Oryza sativa) production in rain-fed farmlands, both of which can occur sequentially during a single crop cycle. SUB1A, an ERF transcription factor found in limited rice accessions, dampens ethylene production and gibberellic acid responsiveness during submergence, economizing carbohydrate reserves and significantly prolonging endurance. Here, we evaluated the functional role of SUB1A in acclimation to dehydration. Comparative analysis of genotypes with and without SUB1A revealed that SUB1A enhanced recovery from drought at the vegetative stage through reduction of leaf water loss and lipid peroxidation and increased expression of genes associated with acclimation to dehydration. Overexpression of SUB1A augmented ABA responsiveness, thereby activating stress-inducible gene expression. Paradoxically, vegetative tissue undergoes dehydration upon desubmergence even though the soil contains sufficient water, indicating that leaf desiccation occurs in the natural progression of a flooding event. Desubmergence caused the upregulation of gene transcripts associated with acclimation to dehydration, with higher induction in SUB1A genotypes. SUB1A also restrained accumulation of reactive oxygen species (ROS) in aerial tissue during drought and desubmergence. Consistently, SUB1A increased the abundance of transcripts encoding ROS scavenging enzymes, resulting in enhanced tolerance to oxidative stress. Therefore, in addition to providing robust submergence tolerance, SUB1A improves survival of rapid dehydration following desubmergence and water deficit during drought.