Flagellar Development in the Protozoan Peranema trichophorum
Flagellar Development in the Protozoan Peranema trichophorum
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原生动物 Peranema trichophorum 的鞭毛发育
DOI：
10.1002/jez.1401640204
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发表时间：
1967
影响因子：
--
通讯作者：
S. Tamm
中科院分区：
文献类型：
--
作者：
S. Tamm
鞭毛长度的变化在鞭毛复制和鞭毛再生的发展过程中的事件进行了研究，在单细胞的无色眼虫鞭毛虫Peranema trichophorum在已知的阶段，在生命周期。用显微操作器操作的钨刀可以任意长度切断前鞭毛，并记录再生过程。
在鞭毛由分裂后细胞再生的过程中，有一个短暂的滞后期，随后是一个减速的伸长。伸长速率和鞭毛长度之间的关系成立，无论初始残端长度。在给定长度的伸长率是更大的分裂前细胞比分裂后细胞。
在细胞分裂时，亲本的前导鞭毛以恒定的速率迅速缩短并消失，同时两个新的前导鞭毛以减速的速率伸长。在整个间期，伸长以较慢的速率继续。
鞭毛在正常细胞周期中的伸长动力学，以及在不同细胞年龄的再生动力学已满意地占了一个数学模型。
本文比较了缩短和伸长，并根据缩短中断某些分裂前细胞再生的发现，讨论了它们之间的关系。
得出的结论是，对鞭毛长度变化的控制至少有一部分在于鞭毛本身。
在各种实验条件下，滞后期保持不变，表明这是一种“伤口愈合反应”。
Changes in flagellar length during the developmental events of flagellar replication and flagellar regeneration were investigated in single cells of the colorless euglenid flagellate Peranema trichophorum at known stages in the life cycle. The leading flagellum could be cut off at any desired length with a tungsten knife operated by a micromanipulator, and the regeneration process recorded photographically.
In the regeneration of a flagellum by a postdivision cell, there is a short lag period followed by an elongation with a deceleratory rate. The relationship between elongation rate and flagellar length holds true regardless of the initial stump length. The elongation rates at given lengths are greater in predivision cells than in postdivision cells.
At cell division, the parental leading flagellum rapidly shortens at a constant rate and disappears, while simultaneously two new leading flagella elongate at a deceleratory rate. Elongation continues at slower rates throughout interphase.
The elongation kinetics of the flagellum during the normal cell cycle, and the kinetics of regeneration at different cell ages have been satisfactorily accounted for by a mathematical model.
Shortening and elongation were compared and the relationship between them was discussed in light of the finding that shortening interrupted regeneration in some predivision cells.
It was concluded that at least part of the control exerted over flagellar length changes resides in the flagellum itself.
The lag period remained the same under a variety of experimental conditions, suggesting that it is a “wound healing response”.