Conservation and mutation of viral interleukin‐10 gene in gastric carcinomas and nasopharyngeal carcinomas
Conservation and mutation of viral interleukin‐10 gene in gastric carcinomas and nasopharyngeal carcinomas
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DOI：
10.1002/jmv.22091
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发表时间：
2011-04
影响因子：
12.7
通讯作者：
Yan Chao;Yong-zheng Jing;Yu-ping Jia;Yun Wang;Chengquan Zhao;B. Luo
中科院分区：
文献类型：
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作者：
Yan Chao;Yong-zheng Jing;Yu-ping Jia;Yun Wang;Chengquan Zhao;B. Luo
EB病毒（EBV）BamH I-C片段编码的病毒白细胞介素-10（vIL-10）的双阅读框1（BCRF 1），与人白细胞介素-10（hIL-10）基因具有高度同源性。蛋白质产物vIL-10与hIL-10具有某些功能特性，主要介导免疫抑制功能。为了解vIL-10基因的变异特征并探讨vIL-10基因变异与EBV相关疾病之间的关系，采用直接测序法分析了中国北方41例EBV相关胃癌（EBVaGC）、83例鼻咽癌活检标本和40例健康供体咽洗液样本中的vIL-10基因。绝大多数EBVaGC、鼻咽癌和咽洗液标本中发现一个沉默突变（c9980 a）（134/164，81.7%）。在部分鼻咽癌和咽拭子分离株的信号肽区发现了两个共有突变（V6 M和G23 S）。这些结果表明，模式B 95 - 8（与B 95 - 8序列相同）是来自中国北方的EBV分离株中的优势型，而模式SPM（仅存在于鼻咽癌和咽喉冲洗分离株中的信号肽突变）似乎与EBV阳性的鼻咽和咽喉上皮细胞更相关。vIL-10的保守性（有一些变异）表明，vIL-10基因对EBV在获得对宿主免疫系统的优势方面起着关键作用。J. Med. Virol. 83：644-650，2011.© 2011 Wiley利斯公司
The Epstein–Barr virus (EBV) BamHI‐C fragment rightward reading frame 1 (BCRF1)‐coded viral interleukin‐10 (vIL‐10), exhibits high homology with human interleukin‐10 (hIL‐10) gene. The protein product vIL‐10, which shares some functional properties with hIL‐10, primarily mediates immunosuppressive functions. To characterize the variations of the vIL‐10 gene and to explore the association between vIL‐10 gene variations and EBV associated diseases, the vIL‐10 gene was analyzed (using direct sequencing) in 41 cases of EBV‐associated gastric carcinoma (EBVaGC), 83 nasopharyngeal carcinoma biopsies, and 40 throat washing samples from healthy donors in Northern China. One silent mutation (c9980a) was observed in the majority of EBVaGC, nasopharyngeal carcinoma and throat washing samples (134/164, 81.7%). Two consensus mutations (V6M and G23S) were identified in the signal peptide region in some nasopharyngeal carcinoma and throat washing isolates. These results indicate that the pattern B95‐8 (identical sequence to B95‐8) is the dominant type among the EBV isolates from Northern China, while the pattern SPM (mutation in the signal peptide present only in nasopharyngeal carcinoma and throat washing isolates) seems more relevant with the EBV‐positive nasopharyngeal and laryngopharyngeal epithelial cells. The conservation of vIL‐10, with a few variations, suggests the critical role of the vIL‐10 gene for EBV in gaining an advantage over the host's immune system. J. Med. Virol. 83:644–650, 2011. © 2011 Wiley‐Liss, Inc.