Expression of Dlx genes during the development of the zebrafish pharyngeal dentition: evolutionary implications
Expression of Dlx genes during the development of the zebrafish pharyngeal dentition: evolutionary implications
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DOI：
10.1111/j.1525-142x.2006.00084.x
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发表时间：
2006-03-01
影响因子：
2.9
通讯作者：
Sire, JY
中科院分区：
文献类型：
--
作者：
Borday-Birraux, V;Van der Heyden, C;Sire, JY
为了研究物种内和物种间牙齿发育的遗传控制的相似性和差异，我们确定了斑马鱼咽齿系发育过程中所有8个Dlx基因的表达模式，并将这些数据与小鼠磨牙发育的数据进行了比较。我们发现（i）dlx 1a和dlx 6a在牙齿中不表达，与它们的小鼠直系同源物Dlx 1和Dlx 6相反;（ii）其他六种斑马鱼Dlx基因的表达在时间和空间上重叠，特别是在早期形态发生期间;（iii）斑马鱼牙列内不同位置和世代的牙齿在表达的基因数量上不同;（iv）斑马鱼Dlx基因之间的表达相似性和差异不明显遵循系统发育和连锁关系;以及（v）斑马鱼和小鼠Dlx直向同源物的表达存在相似性和差异。综上所述，这些结果表明，Dlx基因家族，尽管已经参与了脊椎动物牙齿发育超过4亿年，已经经历了广泛的多样化的表达的个别基因内和之间的牙列。后一种类型的差异可能反映了小鼠相对于斑马鱼的高度专门化的牙列，和/或斑马鱼谱系中的基因组重复，从而促进牙发育过程中Dlx基因功能的重新分布。
In order to investigate similarities and differences in genetic control of development among teeth within and between species, we determined the expression pattern of all eight Dlx genes of the zebrafish during development of the pharyngeal dentition and compared these data with that reported for mouse molar tooth development. We found that (i) dlx1a and dlx6a are not expressed in teeth, in contrast to their murine orthologs, Dlx1 and Dlx6; (ii) the expression of the six other zebrafish Dlx genes overlaps in time and space, particularly during early morphogenesis; (iii) teeth in different locations and generations within the zebrafish dentition differ in the number of genes expressed; (iv) expression similarities and differences between zebrafish Dlx genes do not clearly follow phylogenetic and linkage relationships; and (v) similarities and differences exist in the expression of zebrafish and mouse Dlx orthologs. Taken together, these results indicate that the Dlx gene family, despite having been involved in vertebrate tooth development for over 400 million years, has undergone extensive diversification of expression of individual genes both within and between dentitions. The latter type of difference may reflect the highly specialized dentition of the mouse relative to that of the zebrafish, and/or genome duplication in the zebrafish lineage facilitating a redistribution of Dlx gene function during odontogenesis.