Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts.
Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts.
复制标题
通过直接重编程小鼠成纤维细胞诱导成骨细胞。
DOI：
10.1007/978-1-0716-0655-1_17
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Wu,JoyY
中科院分区：
文献类型：
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作者：
Zhu,Hui;Wu,JoyY
在组织培养皿中，成骨细胞可以来源于间充质干细胞（MSC）和多能干细胞（PSC），包括胚胎干细胞（ESC）和诱导多能干细胞（iPSC）。然而，从PSC分化成骨细胞是耗时且低产量的。与此相反，我们确定了四个成骨转录因子，Runx 2，Osx，Dlx 5和ATF 4，快速，有效地重编程小鼠成纤维细胞来源于2.3 kb I型胶原启动子驱动的绿色荧光蛋白（Col2.3GFP）转基因小鼠诱导成骨细胞（iOBs）。iOB呈现成骨细胞形态，形成矿化结节，并表达Col2.3GFP和成骨细胞分化基因标记。我们的方法提供了一个强大的系统，以快速产生适当的和丰富的成骨细胞的成骨和骨再生研究。
In the tissue culture dish, osteoblast cells can be derived from mesenchymal stem cells (MSCs) and pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). However, differentiation of osteoblasts from PSCs is time-consuming and low yield. In contrast, we identified four osteogenic transcription factors, Runx2, Osx, Dlx5, and ATF4, that rapidly and efficiently reprogram mouse fibroblasts derived from 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP) transgenic mice into induced osteoblast cells (iOBs). iOBs exhibit osteoblast morphology, form mineralized nodules, and express Col2.3GFP and gene markers of osteoblast differentiation. Our method provides a robust system to rapidly generate appropriate and abundant osteoblast cells for osteogenesis and bone regeneration study.