A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L.
A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L.
复制标题
DOI：
10.1007/s004970050088
复制
发表时间：
1997-08-01
影响因子：
--
通讯作者：
Campagne, MMV
中科院分区：
文献类型：
--
作者：
Binarova, P;Hause, G;Campagne, MMV
迄今为止，人们一直认为油菜种子中只有晚期单核小孢子和早期双细胞花粉才有能力诱导离体胚胎发生。在这里，我们描述了在双细胞后期分离的花粉也被诱导经历胚胎发生。通过施加额外的短时间和更严重的热应激处理，DNA合成在生殖和营养细胞核中均开始，但只有营养细胞能够完成细胞周期并进一步分裂。晚期双细胞花粉对胚性诱导处理的反应能力伴随着微管细胞骨架的重排和70 kDa热休克蛋白（HSP 70）的核定位。这些发现证实了早期的观察结果，即胚胎发生的诱导和HSP 70的合成和核定位之间有很强的相关性。
Until now it has been considered that in rape seed (Brassica napus) only late uninucleate microspores and early bicellular pollen are competent for induction of in vitro embryogenesis. Here we describe that pollen isolated at the late bicellular stage call also be induced to undergo embryogenesis. By the application of an additional short and more severe heat stress treatment, DNA synthesis was initialed in both generative and vegetative nuclei, but only vegetative cells were able to complete the cell cycle and to divide further. The ability of late bicellular pollen to respond to embryogenic induction treatment was accompanied by rearrangements of the microtubulular cytoskeleton and by the nuclear localization of 70 kDa heat shock proteins (HSP70). These findings confirm earlier observations that there is a strong correlation between the induction of embryogenesis and the synthesis and nuclear localization of HSP70.