Prep1, a novel functional partner of Pbx proteins
Prep1, a novel functional partner of Pbx proteins
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DOI：
10.1093/emboj/17.5.1423
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发表时间：
1998-03-02
期刊：
影响因子：
11.4
通讯作者：
Blasi, F
中科院分区：
文献类型：
--
作者：
Berthelsen, J;Zappavigna, V;Blasi, F
人转录因子UEF 3在调节尿激酶纤溶酶原激活物（uPA）基因增强子活性中起重要作用，UEF 3 DNA靶位点是多种生长因子和蛋白酶基因启动子的调控元件。在本文中，我们报道了编码一种新的人类同源结构域蛋白的亚基之一的cDNA的克隆，我们将其命名为Prep 1。Prep 1同源结构域属于同源结构域的TALE类，与TGIF和Meis 1蛋白的那些最密切相关，并且像这些一样，识别TGACAG基序。我们进一步鉴定了另一个UEF 3亚基作为Pbx蛋白家族的成员。与已知与Pbx相互作用的其他蛋白质不同，Prep 1与Pbx形成稳定的复合物，而不依赖于DNA结合。Prep 1和Pbx的异源二聚化导致对uPA启动子的TGACAG靶位点的强DNA结合亲和力。总体而言，这些数据表明Prep 1是体内Pbx的稳定细胞内伴侣。
The human transcription factor, UEF3, is important in regulating the activity of the urokinase plasminogen activator (uPA) gene enhancer, The UEF3 DNA target site is a regulatory element in the promoters of several growth factor and protease genes, We reported previously that purified UEF3 is a complex of several subunits, In this paper we report the cloning of the cDNA of one of the subunits which encodes for a novel human homeodomain protein, which we]have termed Prep1, The Prep1 homeodomain belongs to the TALE class of homeodomains, is most closely related to those of the TGIF and Meis1 proteins, and like these, recognizes a TGACAG motif, We further identify the other UEF3 subunit as a member of the Pbx protein family, Unlike other proteins known to interact with Pbx, Prep1 forms a stable complex with Pbx independent of DNA binding. Heterodimerization of Prep1 and Pbx results in a strong DNA binding affinity towards the TGACAG target site of the uPA promoter, Overall, these data indicate that Prep1 is a stable intracellular partner of Pbx in vivo.