Impedance spectroscopy of interfaces, membranes and ultrastructures
Impedance spectroscopy of interfaces, membranes and ultrastructures
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DOI：
10.1016/0302-4598(96)05064-7
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发表时间：
1996-08-01
期刊：
影响因子：
--
通讯作者：
Coster, ACF
中科院分区：
文献类型：
--
作者：
Coster, HGL;Chilcott, TC;Coster, ACF
在过去的世纪，阻抗谱提供了一个非侵入性的手段，表征许多系统的电性能。即使在今天，它也经常为这些系统的详细结构-功能研究提供唯一的非侵入性方法。对于那些在分子水平上发生重要过程的系统，例如与生物和合成膜以及在溶液和各种固体之间形成的界面相关的那些过程（如金属和胶体粒子）。阻抗谱的基本概念是重新-检查和审查的作用，阻抗谱已经发挥了我们的理解细胞和合成膜的发展，细胞生物物理学和一般的离子系统。特别强调了与溶液-电极界面以及未搅拌层有关的问题，这些问题可能困扰生物系统的测量，并在过去导致了许多混乱。描述了一种新的计算机控制的四端数字阻抗谱仪，其在10（-2）到10（5）的频率范围内分别提供0.002%和0.001度的阻抗幅度和相位分辨率Hz和阻抗范围从10到10（9）Ω。我们还描述了传递函数的阻抗色散，当沿负频率轴沿着绘制时，产生具有识别系统基频常数的明显尖峰的“谱”。阻抗谱的这种“控制工程”形式的呈现揭开了这些系统的阻抗分析的神秘面纱。由于系统的扰动而发生的光谱和变化可以很容易地进行评估和解释。
For the past century, impedance spectroscopy has provided a non-invasive means of characterizing the electrical properties of many systems. Even today, it often provides the only non-invasive method for detailed structural-functional studies of these systems. This is especially so of systems in which important processes occur at the molecular level, such as those processes associated with biological and synthetic membranes and interfaces that form between solutions and various solids (e.g. metals and colloid particles).The fundamental concepts of impedance spectroscopy are re-examined and a review is given of the role that impedance spectroscopy has played in the development of our understanding of cellular and synthetic membranes, cell biophysics and ionic systems in general. Special emphasis is given to the problems associated with solution-electrode interfaces, as well as unstirred layers, which can plague measurements on biological systems and have led to much confusion in the past,A description is given of a new computer-controlled, four-terminal digital impedance spectrometer, which provides resolutions in impedance magnitude and phase of 0.002% and 0.001 degrees respectively over a frequency range of 10(-2) to 10(5) Hz and for impedances ranging from 10 to 10(9) Omega.We also describe impedance dispersions in terms of transfer functions which, when plotted along the negative frequency axis, yield ''spectra'' with distinct sharp peaks that identify fundamental frequency constants of the system. This ''control engineering'' form of presentation of impedance spectra demystifies the impedance analyses of these systems. The spectra and changes in these which occur as a result of perturbations to the system can be readily assessed and interpreted.