Distribution and projections of neurons with immunoreactivity for both gastrin-releasing peptide and bombesin in the guinea-pig small intestine
Distribution and projections of neurons with immunoreactivity for both gastrin-releasing peptide and bombesin in the guinea-pig small intestine
复制标题
豚鼠小肠中对胃泌素释放肽和铃蟾肽具有免疫反应性的神经元的分布和投射
DOI：
10.1007/bf00217852
复制
发表时间：
2004
影响因子：
3.6
通讯作者：
T. Moody
中科院分区：
文献类型：
--
作者：
M. Costa;J. Furness;N. Yanaihara;C. Yanaihara;T. Moody
本研究利用合成肽的抗体定位于豚鼠小肠和腹腔神经节神经中的蛙皮素样和胃泌素释放肽（GRP）样免疫反应。抗蛙皮素血清（预孵育以避免与P物质交叉反应）和抗GRP血清显示了相同的神经元群体。用蛙皮素预孵育抗蛙皮素血清可消除神经中的免疫反应性，而用蛙皮素或GRP的14-27 C-末端吸收抗GRP血清仅降低免疫反应性。免疫反应阳性神经分布于肌间神经丛、环肌、粘膜下神经丛和腹腔神经节。在肌间神经节（占所有神经元的3.2%）中发现微弱反应的神经细胞体，但在粘膜下神经节中未发现。切断肌间神经丛的所有上行和下行通路后，肌间神经丛、环肌（包括深肌丛）和肛侧粘膜下神经丛的反应性终末消失。切断肠系膜神经后，肠壁无明显变化，但腹腔神经节内反应纤维消失。推测肌间神经节中GRP/蛙皮素免疫反应阳性神经元胞体从肌间神经丛向肛门更远处的肌间神经节投射（平均长度12 mm），肛门至环肌（平均长度9 mm），肛门至粘膜下神经节（平均长度13 mm）和肠外至腹腔神经节。肠壁中的反应性神经元代表可能参与控制运动和协调其它肠功能的肠神经元的独特群体。
SummaryBombesin-like and gastrin-releasing peptide (GRP)-like immunoreactivities were localized in nerves of the guinea-pig small intestine and celiac ganglion with the use of antibodies raised against the synthetic peptides. The anti-bombesin serum (preincubated to avoid cross reactivity with substance P) and the anti-GRP serum revealed the same population of neurons. Preincubation of the antibombesin serum with bombesin abolished the immunoreactivity in nerves while absorption of the anti-GRP serum with either bombesin or the 14–27 C-terminal of GRP only reduced the immunoreactivity. The immunoreactivity was abolished by incubation with GRP 1–27.Immunoreactive nerves were found in the myenteric plexus, circular muscle, submucous plexus and in the celiac ganglion. Faintly reactive nerve cell bodies were found in the myenteric ganglia (3.2% of all neurons) but not in submucous ganglia. After all ascending and descending pathways in the myenteric plexus had been cut, reactive terminals disappeared in the myenteric plexus, circular muscle (including the deep muscular plexus) and the submucous plexus on the anal side. After the mesenteric nerves were cut no changes were observed in the intestinal wall but the reactive fibres in celiac ganglia disappeared. It is deduced that GRP/bombesin-immunoreactive nerve cell bodies in myenteric ganglia project from the myenteric plexus to other myenteric ganglia situated further anally (average length 12 mm), anally to the circular muscle (average length 9 mm), anally to submucous ganglia (average length 13 mm) and external to the intestine to the celiac ganglia.It is concluded that the GRP/bombesin-reactive neurons in the intestinal wall represent a distinct population of enteric neurons likely to be involved in controlling motility and in the coordination of other intestinal functions.