No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population.
No evidence of correlation between the single nucleotide polymorphism of DNMT3B promoter and gastric cancer risk in a Japanese population.
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DOI：
10.3892/or.14.5.1151
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发表时间：
2005-11
期刊：
影响因子：
4.2
通讯作者：
P. Aung;Shunji Matsumura;K. Kuraoka;Kyoko Kunimitsu;Kazuhiro Yoshida;K. Matsusaki;H. Nakayama;W. Yasui
中科院分区：
文献类型：
--
作者：
P. Aung;Shunji Matsumura;K. Kuraoka;Kyoko Kunimitsu;Kazuhiro Yoshida;K. Matsusaki;H. Nakayama;W. Yasui
DNA甲基化是人类主要的表观遗传修饰，异常的DNA甲基化可能通过沉默一些肿瘤抑制基因在各种癌症的发展中发挥重要作用。DNMT3B是基因组甲基化模式建立和维持所必需的。DNMT3B启动子的-149 C/T单核苷酸多态性（SNP）已被确定。该SNP影响DNMT3B启动子功能，其中T等位基因比C等位基因具有更大的活性，并且与肺癌风险增加有关。我们的研究目的是探讨DNMT3B启动子多态性与胃癌发生发展的相关性。我们利用PCR-RFLP和测序技术分析了152例胃癌患者和247例日本人群的DNMT3B启动子SNP，并研究了DNMT3B基因型与病例间临床病理参数的关系。胃癌患者与对照组在DNMT3B基因启动子转录起始位点-149 bp处的靶位点未发现等位基因差异。所有胃癌患者和对照组均检测到T/T基因型。我们得出结论，在日本人群中，DNMT3B启动子的SNP与胃癌风险之间没有关联。
DNA methylation is a major epigenetic modification in humans, and aberrant DNA methylation may play an important role in the development of various cancers through the silencing of some tumor suppressor genes. DNMT3B is required for the establishment and maintenance of genomic methylation patterns. The -149 C/T single nucleotide polymorphism (SNP) in the promoter of DNMT3B has been identified. This SNP influences DNMT3B promoter function, with the T allele having greater activity than the C allele, and is associated with an increased risk of lung cancer. The purpose of our study was to investigate the correlation between the DNMT3B promoter polymorphism and the development and progression of gastric cancer. We analyzed the SNP of the DNMT3B promoter in 152 gastric cancer patients and 247 controls from a Japanese population using PCR-RFLP and sequencing analysis, and also studied the association between the genotypes of DNMT3B and clinicopathological parameters among cases. Allelic difference was not found between gastric cancer patients and control subjects at the target site, -149 bp from the transcriptional start site in the DNMT3B gene promoter. Only the T/T genotype was detected in all gastric cancer patients and control subjects. We concluded that there was no association between SNP of the DNMT3B promoter and gastric cancer risk in a Japanese population.