Prokaryotic Recombinant Hemagglutinin-Neuraminidase Protein Enhances the Humoral Response and Efficacy of Commercial Newcastle Disease Vaccines in Chickens
Prokaryotic Recombinant Hemagglutinin-Neuraminidase Protein Enhances the Humoral Response and Efficacy of Commercial Newcastle Disease Vaccines in Chickens
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DOI：
10.1637/8965-062009-reg.1
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发表时间：
2010-03-01
期刊：
影响因子：
1.4
通讯作者：
Chu, Chun-Yen
中科院分区：
文献类型：
--
作者：
Lee, Jai-Wei;Huang, Ji-Ping;Chu, Chun-Yen
新城疫病毒（NDV）的血凝素-神经氨酸酶（HN）蛋白在新城疫病（ND）的发病过程中起重要作用。通过直接注射含有HN基因的重组病毒或杆状病毒表达系统制备的重组HN蛋白已被用于鸡对NDV的免疫。本研究通过原核表达系统表达了60.4 kda的rHN，并将其配制成ND疫苗。将rHN （10 μ g/ml）加入常规ND灭活疫苗中，显著（P < 0.05）提高了无特定病原体或商品鸡血清血凝抑制抗体的滴度。此外，将rHN蛋白配制成ND+IC（传染性鼻炎）二价疫苗或ND+IC+FC（禽霍乱）多价疫苗，免疫后的鸡在NDV攻毒后的保护率从类似的80% ~ 90%提高到100%。我们的数据表明，包含由经济的原核表达系统产生的rHN蛋白可以增强传统和多价ND灭活疫苗的免疫原性。这种方法可用于提高目前在家禽业中使用的新城疫疫苗的效力。
The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) plays an important role in the pathogenesis of Newcastle disease (ND). Recombinant HN (rHN) protein, produced either by direct injection of recombinant viruses containing HN gene or baculovirus expression systems, has been used to elicit immunity against NDV in chickens. In the present study, a 60.4-kDa rHN was expressed by a prokaryotic expression system and formulated into ND vaccines. Inclusion of rHN (10 mu g/ml) into conventional, inactivated ND vaccines significantly (P < 0.05) increased the titer of serum hemagglutination-inhibition Ab in specific-pathogen-free or commercial chickens. Furthermore, when the rHN protein was formulated into ND+IC (infectious coryza) bivalent or ND+IC+FC (fowl cholera) multivalent vaccines, the protection rate of immunized chickens increased from similar to 80%-90% to 100% after being challenged by a velogenic strain of NDV. Our data indicated that inclusion of rHN protein produced by an economical prokaryotic expression system could enhance the immunogenicity of traditional and multivalent inactivated ND vaccines. This approach may be adapted to improve the efficacy of ND vaccines currently used in the poultry industry.