First isolation and genomic characterization of bovine parechovirus from faecal samples of cattle in Japan
First isolation and genomic characterization of bovine parechovirus from faecal samples of cattle in Japan
复制标题
日本首次从牛粪便样本中分离出牛副肠孤病毒并进行基因组表征
DOI：
10.1099/jgv.0.001718
复制
发表时间：
2022
影响因子：
3.8
通讯作者：
Nagai Makoto
中科院分区：
文献类型：
--
作者：
Oba Mami;Sakaguchi Shoichi;Wu Hong;Fujioka Yoshihiko;Takemae Hitoshi;Oki Hisako;Kawai Megumi;Shiokawa Mai;Aoki Hiroshi;Fukase Yuka;Madarame Hiroo;Nakano Takashi;Mizutani Tetsuya;Nagai Makoto
用MA-104细胞在第三次盲传时从一头腹泻奶牛的粪便中分离到一种新的小核糖核酸病毒。该病毒命名为Den1/2021/JPN，使用来自细胞培养上清液的总RNA通过深度测序进行完全测序。Den1/2021/JPN的基因组具有标准的小核糖核酸病毒基因组组织，具有保守的小核糖核酸病毒基序。5 ′非翻译区含有一个II型核糖体内部进入位点。Den1/2021/JPN与最近在公开数据库中发现的名为cow/2018/4的牛双埃柯病毒（Bo_ParV）关系最密切。系统进化分析和序列比对结果表明，Den1/2021/JPN和Bo_ParV cow/2018/4与双埃柯病毒属（Parechovirus）病毒属（Par 本研究首次报道了Bo_ParV的分离。Den1/2021/JPN和Bo_ParV cow/2018/4，它们是副肠孤病毒属新种的候选者。
A novel picornavirus was isolated from the faeces of a diarrhoeic cow using MA-104 cells at the third blind passage. This virus, named Den1/2021/JPN, was completely sequenced using total RNA from the cell culture supernatant by deep sequencing. The genome of Den1/2021/JPN had a standard picornavirus genome organisation with conserved picornaviral motifs. The 5′ untranslated region harboured a type-II internal ribosomal entry site. Den1/2021/JPN was most closely related to a bovine parechovirus (Bo_ParV) named cow/2018/4, which has been recently identified in publicly available databases. Phylogenetic analyses and pairwise sequence comparison revealed that Den1/2021/JPN and Bo_ParV cow/2018/4 clustered with parechoviruses and were most closely related toParechovirus Eidentified in birds of prey, exhibiting nucleotide sequence similarity of 64.2–64.5 %, 58.6–59.7 % and 66.3–66.4 % in the polyprotein, P1 and 2C+3 CD coding regions, respectively. This study presents the first report on the isolation of Bo_ParV. Den1/2021/JPN and Bo_ParV cow/2018/4, which are candidates for a novel species in the genusParechovirus.