A dominant negative form of transcription activator mTFE3 created by differential splicing.
A dominant negative form of transcription activator mTFE3 created by differential splicing.
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通过差异剪接产生的转录激活子 mTFE3 的显性失活形式。
DOI：
10.1126/science.1840705
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Calame,K
中科院分区：
文献类型：
--
作者：
Roman,C;Cohn,L;Calame,K
转录因子 E3 (mTFE3) 是一种鼠类转录激活剂，可与免疫球蛋白重链基因的内含子增强子结合。 mTFE3 信使 RNA (mRNA) 的天然剪接产物缺乏 105 个编码激活结构域的核苷酸；不同组织中长mRNA和截短mRNA的绝对量和相对量各不相同。细胞被编码两种 mRNA 形式的互补 DNA 共转染，其数量与不同细胞中每种 mRNA 的数量相对应。 mRNA 截短形式的亚化学计量的微小变化会影响 mTFE3 活性的反式显性负调节。这些发现确定了差异剪接在转录因子活性调节中的功能。
Transcription factor E3 (mTFE3) is a murine transcription activator that binds to the intronic enhancer of the immunoglobulin heavy chain gene. A naturally occurring splice product of mTFE3 messenger RNA (mRNA) lacked 105 nucleotides that encode an activation domain; both absolute and relative amounts of long and truncated mRNAs varied in different tissues. Cells were cotransfected with complementary DNAs that encoded the two mRNA forms in amounts that corresponded to the amounts of each mRNA found in different cells. Small changes in substoichiometric amounts of the truncated form of mRNA effected trans-dominant negative modulation of mTFE3 activity. These findings identify a function for differential splicing in the regulation of transcription factor activity.