Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models.
Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models.
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人类和小鼠体外脂肪形成模型的单细胞转录组数据集。
DOI：
10.1101/2023.03.27.534456
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Knowles,JoshuaW
中科院分区：
文献类型：
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作者：
Li,Jiehan;Jin,Christopher;Gustafsson,Stefan;Rao,Abhiram;Wabitsch,Martin;Park,ChongY;Quertermous,Thomas;Bielczyk-Maczynska,Ewa;Knowles,JoshuaW
脂肪生成是脂肪特异性前体细胞(前体脂肪细胞)分化为脂肪细胞的过程，脂肪细胞执行脂肪组织的关键代谢功能，包括葡萄糖摄取、能量储存和脂肪因子分泌。一些细胞系被常规用于研究脂肪形成的分子调控，特别是永生化小鼠3T3-L1系和原代人类Simpson-Golabi-Behmel综合征(SGBs)系。然而，在这些模型中，在脂肪形成之前和过程中转录变化的细胞间的可变性还没有被很好地理解。在这里，我们提供了一个单细胞RNA测序(scRNA-Seq)数据集，收集在3T3-L1和SGBs细胞成脂分化之前和过程中。为了最大限度地减少实验变异的影响，我们混合了3T3-L1和SGBs细胞，并使用计算分析来分离小鼠和人类细胞的转录。在这两种模型中，脂肪生成导致三个细胞团的出现，分别对应于前脂肪细胞、早期和成熟脂肪细胞。这些数据为在人类和小鼠脂肪形成的体外模型中广泛使用的这些方法的比较研究提供了基础，并为这一过程中细胞间的变异性提供了基础。
Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study the molecular regulation of adipogenesis, in particular the immortalized mouse 3T3-L1 line and the primary human Simpson-Golabi-Behmel syndrome (SGBS) line. However, the cell-to-cell variability of transcriptional changes prior to and during adipogenesis in these models is not well understood. Here, we present a single-cell RNA-Sequencing (scRNA-Seq) dataset collected before and during adipogenic differentiation of 3T3-L1 and SGBS cells. To minimize the effects of experimental variation, we mixed 3T3-L1 and SGBS cells and used computational analysis to demultiplex transcriptomes of mouse and human cells. In both models, adipogenesis results in the appearance of three cell clusters, corresponding to preadipocytes, early and mature adipocytes. These data provide a groundwork for comparative studies on these widely usedin vitromodels of human and mouse adipogenesis, and on cell-to-cell variability during this process.