Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)
Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)
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DOI：
10.1007/s00122-024-04569-1
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发表时间：
2024-03-01
影响因子：
5.4
通讯作者：
Wells，Rachel
中科院分区：
文献类型：
--
作者：
Jacott，Catherine N.;Schoonbeek，Henk-jan;Wells，Rachel
Key messageUsing associative transcriptomics，our study identified genes conferring resistance to four different fungal pathogens in crops，emphasizing key genetic determinants of multi-pathogens resistance.AbstractCrops are affected by several pathogens，but these are also鲜少studied in parallel to identify common and unique genetic factors controlling diseases.广谱定量抗病性（QDR）是作物育种所需要的，因为它赋予对几种病原体物种的抗性。在此，我们利用关联转录组学（AT）技术鉴定了与甘蓝型油菜对四种不同真菌病原菌（Alternaria brassicicicola，Botrytis cinerea，Pyrenopeziza brassicae和Verticillium longisporum）的组成型QDR相关的候选基因位点，但没有发现与生活方式不同的真菌病原菌的广谱QDR相关的任何共有位点。相反，我们观察到QDR依赖于病原体的生活方式-半活体营养型和坏死营养型病原体具有不同的QDR反应和相关位点，包括一些与早期免疫相关的位点。此外，我们确定了一个基因组缺失与耐V。longisporum和潜在的广谱QDR。这是第一次AT已被用于几个病理系统，同时确定参与广谱QDR的宿主遗传位点。我们强调组成型表达的候选位点的广谱QDR没有拮抗作用的敏感性，其他病原体的研究作为候选作物育种。总之，这项研究代表了我们对B中广谱QDR的理解的进步。napusand是科学界的重要资源。
Key messageUsing associative transcriptomics, our study identifies genes conferring resistance to four diverse fungal pathogens in crops, emphasizing key genetic determinants of multi-pathogen resistance.AbstractCrops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated withBrassica napusconstitutive QDR to four contrasting fungal pathogens:Alternaria brassicicola,Botrytis cinerea,Pyrenopeziza brassicae,andVerticillium longisporum.We did not identify any shared loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen—hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance toV. longisporumand potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutive expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents an advancement in our understanding of broad-spectrum QDR inB. napusand is a significant resource for the scientific community.