Induced production of emericellamides A and B from the marine-derived fungus Emericella sp in competing co-culture
Induced production of emericellamides A and B from the marine-derived fungus Emericella sp in competing co-culture
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DOI：
10.1021/np060381f
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发表时间：
2007-04-01
影响因子：
5.1
通讯作者：
Fenical, William
中科院分区：
文献类型：
--
作者：
Oh, Dong-Chan;Kauffman, Christopher A.;Fenical, William
在与海洋放线菌沙利菌（Salinispora arenicola）共培养过程中，观察到海洋源真菌Emericella sp.诱导emericellamides A和B（1,2）的产生。这些含有3-羟基-2,4-二甲基癸酸和3-羟基-2,4,6-三甲基十二酸的环状沉积肽的平面结构通过化学和光谱相结合的方法进行了鉴定。采用Marfey法、j型构型分析和改进的Mosher法确定了氨基酸的绝对构型和侧链上手性中心的绝对构型。Emericellamides A和B对耐甲氧西林金黄色葡萄球菌表现出适度的抗菌活性，MIC值分别为3.8 μ M和6.0 μ M。
Induction of the production of emericellamides A and B (1, 2), by the marine-derived fungus Emericella sp., was observed during co-culture with the marine actinomycete Salinispora arenicola. The planar structures of these new cyclic depsipeptides, which incorporate 3-hydroxy-2,4-dimethyldecanoic acid and 3-hydroxy-2,4,6-trimethyldodecanoic acid, were assigned by combined chemical and spectral methods. The absolute configurations of the amino acids, and those of the chiral centers on the side chain, were established by application of the Marfey's method, by J-based configuration analysis, and by application of the modified Mosher method. Emericellamides A and B show modest antibacterial activities against methicillin-resistant Staphylococcus aureus with MIC values of 3.8 and 6.0 mu M, respectively.