CHANGES IN THE COLLAGENOUS MATRIX OF THE AGING HUMAN LAMINA-CRIBROSA
CHANGES IN THE COLLAGENOUS MATRIX OF THE AGING HUMAN LAMINA-CRIBROSA
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DOI：
10.1136/bjo.79.4.368
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发表时间：
1995-04-01
影响因子：
4.1
通讯作者：
DUANCE, VC
中科院分区：
文献类型：
--
作者：
ALBON, J;KARWATOWSKI, WSS;DUANCE, VC
目的-研究人筛板胶原基质生化成分的年龄相关性变化。分析了人类筛板（3周到92岁）的胶原蛋白溶解度（n=58）、总胶原蛋白含量（n=46）、胶原蛋白类型比例（n=38），和胶原交联（n=30），使用羟脯氨酸分析，溴化氰消化后的肽扫描光密度测定法，和高效液相chromatography.Results-Age相关的变化包括总胶原的增加和筛板内的III型胶原的比例下降。胶原交联吡啶啉以低水平存在，但没有表现出随年龄增长的趋势。一个与年龄相关的增加被发现在pentosidine，一个先进的glycationproduct.Conclusion-These胶原蛋白组合物的变化意味着筛板的机械性能的改变，导致在一个更硬，弹性结构随着年龄的增长。这种结构上的改变可能导致老年人对慢性开角型青光眼轴突损伤的易感性增加。
Aims-The age-related changes in the biochemical composition of the collagenous matrix of the human lamina cribrosa were investigated.Methods-An age range (3 weeks to 92 years old) of human laminae cribrosae, dissected free of any surrounding structures which contained collagen, were analysed for collagen solubility (n=58) total collagen content (n=46), proportion of collagen types (n=38), and collagen cross linking (n=30), using hydroxyproline analysis, scanning densitometry of peptides after cyanogen bromide digestion, and high performance liquid chromatography, respectively.Results-Age-related changes included an increase in total collagen and a decrease in the proportion of type III collagen within the lamina cribrosa. The collagen cross link pyridinoline was present at low levels, but demonstrated no trend with age. An age-related increase was found in pentosidine, an advanced glycation product.Conclusion-These changes in collagen composition imply that the mechanical properties of the lamina cribrosa are altered, resulting in a stiffer, less resilient structure with age. Such alterations in structure may contribute to the increased susceptibility of the elderly to axonal damage in chronic open angle glaucoma.