Osmotic stress, crowding, preferential hydration, and binding: A comparison of perspectives
Osmotic stress, crowding, preferential hydration, and binding: A comparison of perspectives
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DOI：
10.1073/pnas.97.8.3987
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发表时间：
2000-04-11
影响因子：
11.1
通讯作者：
Rau, DC
中科院分区：
文献类型：
--
作者：
Parsegian, VA;Rand, RP;Rau, DC
最近有很多混乱的相对优点不同的方法，渗透胁迫，优先相互作用，拥挤，来描述溶质对大分子构象和反应的间接影响。为了加强对测量的所有解释，并防止进一步不必要的概念或语言混乱，我们在这里展示了如何协调不同的观点。我们的方法是通过吉布斯-迪昂关系，通用的限制的方式，它是可能改变的温度，压力，和化学势的几个组成部分在任何物理定义的系统。从这个通用的吉布斯-迪昂方程中，可以看出不同观点的等价性，甚至可以表明不同方法所使用的更专门的方程的精确同一性。
There has been much confusion recently about the relative merits of different approaches, osmotic stress, preferential interaction, and crowding, to describe the indirect effect of solutes on macromolecular conformations and reactions. To strengthen all interpretations of measurements and to forestall further unnecessary conceptual or linguistic confusion, we show here how the different perspectives all can be reconciled. Our approach is through the Gibbs-Duhem relation, the universal constraint on the number of ways it is possible to change the temperature, pressure, and chemical potentials of the several components in any thermodynamically defined system. From this general Gibbs-Duhem equation, it is possible to see the equivalence of the different perspectives and even to show the precise identity of the more specialized equations that the different approaches use.