MOLECULAR-CLONING OF CDNA-ENCODING MOUSE CDC21 AND CDC46 HOMOLOGS AND CHARACTERIZATION OF THE PRODUCTS - PHYSICAL INTERACTION BETWEEN P1(MCM3) AND CDC46 PROTEINS
MOLECULAR-CLONING OF CDNA-ENCODING MOUSE CDC21 AND CDC46 HOMOLOGS AND CHARACTERIZATION OF THE PRODUCTS - PHYSICAL INTERACTION BETWEEN P1(MCM3) AND CDC46 PROTEINS
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DOI：
10.1093/nar/23.12.2097
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发表时间：
1995-06-25
影响因子：
14.9
通讯作者：
SUGIMOTO, K
中科院分区：
文献类型：
--
作者：
KIMURA, H;TAKIZAWA, N;SUGIMOTO, K
两个新的小鼠基因编码的蛋白质，属于酵母微型染色体维持（MCM）蛋白家族，这是参与启动DNA复制，分离和它们的核苷酸序列进行了测定。它们是推定的CDC 46/MCM 5同源物和推定的cdc 21同源物。在该家族成员之间获得了约30%的氨基酸同一性，并且在推定的小鼠和酵母同源物之间获得了>40%的氨基酸同一性。这些基因的表达是细胞周期特异性的晚期G（1）到S期。免疫化学分析显示小鼠P1 MCM 3和CDC 46蛋白之间存在物理相互作用。这些结果表明，MCM蛋白的功能协调DNA复制。
Two new mouse genes encoding proteins that belong to the yeast minichromosome maintenance (MCM) protein family, which is involved in the initiation of DNA replication, were isolated and their nucleotide sequence was determined. They were a putative CDC46/MCM5 homolog and a putative cdc21 homolog. About 30% amino acid identity was obtained between members in the family, and >40% between the putative mouse and yeast homologs. The expression of these genes was cell-cycle specific at the late G(1) to S phase. Immunochemical analyses showed the physical interaction between mouse P1MCM3 and CDC46 protein. These results suggest that MCM proteins function in co-ordination for DNA replication.