PROINFLAMMATORY EFFECTS OF LOCAL ABDOMINAL IRRADIATION ON RAT GASTROINTESTINAL-TRACT
PROINFLAMMATORY EFFECTS OF LOCAL ABDOMINAL IRRADIATION ON RAT GASTROINTESTINAL-TRACT
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DOI：
10.1007/bf01536261
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发表时间：
1989-03-01
影响因子：
3.1
通讯作者：
HARDING, RK
中科院分区：
文献类型：
--
作者：
BUELL, MG;HARDING, RK
虽然炎症过程在电离辐射后胃肠道迟发性改变的发生中的作用已被广泛研究，但很少有研究集中在辐射后即刻是否存在急性炎症反应。因此，我们研究了在大鼠腹部局部应用10Gy钴-60照射是否在最初的24小时内导致肠道的变化，这与急性炎症反应一致。在胃、小肠和结肠，局部照射导致照射后4-8小时内组织内血浆的积聚显著增加。在所有被检查的组织中，这种组织血浆容量的增加，表明微血管通透性的增加，一直持续到24小时评估期结束。与此相伴随的是组织红细胞体积持续的一过性增加，提示血管扩张。特别值得注意的是，在照射后2至12小时内，还观察到粘膜中性粒细胞数量显著增加。粘膜中性粒细胞的这种升高在小肠和结肠的隐窝或深层粘膜区域特别明显，并且始终先于血管扩张和通透性增强。此外，这些病理生理改变发生在黏膜的组织学变化与黏膜微循环受损(即固有层水肿和上皮下小泡形成)相一致的时候。这些结果支持这样的假设，即在腹部照射后的前24小时内，肠道内发生了炎症反应。这样的变化可能会进一步加剧电离辐射造成的损害。
Although the role of inflammatory processes in the genesis of late changes in the gastrointestinal tract following exposure to ionizing irradiation has been extensively studied, few studies have concentrated on the presence of an acute inflammatory response in the period immediately following radiation. We therefore examined, in rats, whether the local application of 10 Gy cobalt-60 irradiation to the abdomen led to changes in the gut within the first 24 hr that were consistent with an acute inflammatory response. In stomach, small intestine, and colon, local irradiation led to a significant increase in the accumulation of plasma within the tissue by 4-8 hr following irradiation. This increase in tissue plasma volume, indicative of an increased microvascular permeability, was then sustained until the end of the 24-hr assessment period in all tissues examined. Concurrent with this was a consistent transient increase in tissue red blood cell volume, suggestive of vasodilation. Of particular note, a significant increase in the number of mucosal neutrophils was also observed between 2 and 12 hr following irradiation. This elevation in mucosal neutrophils was particularly marked in the pericryptal or deep mucosal regions of small intestine and colon and consistently preceded the vasodilation and enhanced permeability. Furthermore these pathophysiological alterations occurred at a time when histological changes in the mucosa consistent with an impaired mucosal microcirculation (ie, edema of the lamina propria and subepithelial bleb formation) were present. These results support the hypothesis that an inflammatory response occurs in the gut during the first 24 hr following abdominal irradiation. Such changes may then further exacerbate the damage initiated by the ionizing radiation.