Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension
Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension
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DOI：
10.1016/j.jmbbm.2022.105326
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发表时间：
2022-06-29
影响因子：
3.9
通讯作者：
Matsumoto, Takeo
中科院分区：
文献类型：
--
作者：
Fan, Yong;Wang, Junfeng;Matsumoto, Takeo
细胞核的变形可能导致染色质的分散，最终增强转录、翻译和蛋白质表达。如果这种情况发生在高血压动脉，由高血压引起的平滑肌细胞(SMC)核过度伸展可能会引起管壁增厚。在本实验中，我们测量了不同方向牵拉的兔胸主动脉中SMC核的变形。将0.2 mm厚的薄试件在垂直于其轴向和周向的方向上切片，分别在周向和轴向上拉伸。肌动蛋白细丝(AF)网络的变形与整个组织的变形相似，而细胞核的变形明显小于其他组织。值得注意的是，当组织在轴向拉伸时，细胞核很少变形。一个新的细胞模型，其中细胞核通过房颤网络连接到细胞外基质，成功地解释了细胞核对轴向拉伸的相对无反应。已有研究指出，高血压时动脉壁周向应力保持恒定，而轴向应力不变。在这项研究中，细胞核对轴向拉伸的相对无反应解释了这种现象。
The deformation of the cell nucleus may cause dispersion of chromatin and eventually enhance transcription, translation, and protein expression. If this happens in the hypertensive artery, an excessive stretch of smooth muscle cell (SMC) nuclei caused by hypertension may provoke wall thickening. Here, we measured defor-mation of SMC nuclei in rabbit thoracic aortas stretched in different directions. Thin 0.2-mm-thick specimens were sliced in the direction perpendicular to their axial and circumferential directions, and stretched in the circumferential and axial directions, respectively. The deformation of the actin filament (AF) network was similar to that of the whole tissue, whereas the deformation of the nucleus was significantly smaller than the others. Notably, the nucleus seldom deformed when the tissue was stretched in the axial direction. A novel cell model in which the nucleus is connected to the extracellular matrix via the AF network successfully explained the relative unresponsiveness of the nucleus to the axial stretch. It has been pointed out that stress is maintained constant in the circumferential direction but not in the axial direction in the artery wall during hypertension. The relative unresponsiveness of the nucleus to the axial stretch represented in this study explains this phenomenon.