Graphene-modified Interdigitated Array Electrode: Fabrication, Characterization, and Electrochemical Immunoassay Application
Graphene-modified Interdigitated Array Electrode: Fabrication, Characterization, and Electrochemical Immunoassay Application
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DOI：
10.2116/analsci.29.55
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发表时间：
2013-01
影响因子：
1.6
通讯作者：
Y. Ueno;K. Furukawa;K. Hayashi;M. Takamura;H. Hibino;E. Tamechika
中科院分区：
文献类型：
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作者：
Y. Ueno;K. Furukawa;K. Hayashi;M. Takamura;H. Hibino;E. Tamechika
我们开发了一种用还原氧化石墨烯修饰的互指阵列金电极（Au-IDA）的新工艺。在这个过程中，我们在微米级电极的金表面涂上氧化石墨烯（GO），在还原和提升过程之前，以避免在还原后被导电的氧化石墨烯薄片短路。然后，我们研究了制备的电极rGO/Au-IDA的基本电化学活性，主要是对氨基酚（p AP），因为p AP是一种很好的电化学免疫测定探针。伏安图显示，密度更大的还原氧化石墨烯为p - AP提供了更好的电极反应性。我们证实，还原氧化石墨烯/Au-IDA在阳极和阴极之间建立了氧化还原循环，这比使用单一电极产生更灵敏的检测。作为使用rGO/Au-IDA的电化学免疫分析的一个应用，我们展示了在人类唾液中发现的应激标志物皮质醇水平的定量检测。
We have developed a new procedure for fabricating interdigitated array gold electrodes (Au-IDA) modified with reduced graphene oxide (rGO). In this procedure, we coated the gold surface of the micrometer order electrodes with graphene oxide (GO) prior to the reduction and the lift-off processes to avoid short-circuiting the pair of electrodes by conductive rGO flakes after the reduction. We then studied the basic electrochemical activity of the prepared electrodes, rGO/Au-IDA, mainly on p -aminophenol ( p AP), because p AP is a good probe for an electrochemical immunoassay. The voltammograms showed that denser rGO provides better electrode reactivity for p AP. We confirmed that redox cycling between the anode and cathode at the rGO/Au-IDA was established, which yields more sensitive detection than with a single electrode. As one application of the electrochemical immunoassay using the rGO/Au-IDA, we demonstrated the quantitative detection of cortisol, a stress marker, at levels found in human saliva.