TRANSCRIPTIONAL REGULATION OF COORDINATE CHANGES IN FLAGELLAR MESSENGER-RNAS DURING DIFFERENTIATION OF NAEGLERIA-GRUBERI AMEBAE INTO FLAGELLATES
TRANSCRIPTIONAL REGULATION OF COORDINATE CHANGES IN FLAGELLAR MESSENGER-RNAS DURING DIFFERENTIATION OF NAEGLERIA-GRUBERI AMEBAE INTO FLAGELLATES
复制标题
DOI：
10.1128/mcb.8.6.2280
复制
发表时间：
1988-06-01
影响因子：
5.3
通讯作者：
WALSH, CJ
中科院分区：
文献类型：
--
作者：
LEE, JH;WALSH, CJ
利用核启动技术研究了格鲁伯耐格里阿米巴在鞭毛分化过程中鞭毛基因的转录速率。轴丝蛋白α-mRNA的合成和β-微管蛋白和鞭毛钙调蛋白，以及协调调节的poly（A）+ RNA，编码一种未鉴定的蛋白质，表现出平均22倍的瞬时增加。两个poly（A）+RNA的合成速率在阿米巴和鞭毛虫中是很低的，直到鞭毛虫基因的转录开始下降，此时在阿米巴中发现的RNA的合成增加了3至10倍。所观察到的转录速率的变化可以定量解释鞭毛mRNA浓度在分化过程中增加20倍。鞭毛钙调蛋白基因的数据表明非微管蛋白轴丝蛋白的转录调控。这些数据还证明了在分化过程中至少有两种转录调控程序，并提出了一种有趣的可能性，即鞭毛轴丝的近200种不同蛋白质中的一些重要部分在N. gruberi amestrium形成可见的鞭毛。
The nuclear run-on technique was used to measure the rate of transcription of flagellar genes during the differentiation of Naegleria gruberi amebae into flagellates. Synthesis of mRNAs for the axonemal proteins .alpha.- and .beta.-tubulin and flagellar calmodulin, as well as a coordinately regulated poly(A)+ RNA that codes for an unidentified protein, showed transient increases averaging 22-fold. The rate of synthesis of two poly(A)+ RNAs common to amebae and flagellates was low until the transcription of the flagellar genes began to decline, at which time synthesis of the RNAs found in amebae increased 3- to 10-fold. The observed changes in the rate of transcription can account quantitatively for the 20-fold increase in flagellar mRNA concentration during the differentiation. The data for the flagellar calmodulin gene demonstrate transcriptional regulation for a nontubulin axonemal protein. The data also demonstrate at least two programs of transcriptional regulation during the differentiation and raise the intriguing possibility that some significant fraction of the nearly 200 different proteins of the flagellar axoneme is transcriptionally regulated during the 1 h it takes N. gruberi amebae to form visible flagella.