LONG-TERM INCREASE IN THE LEVELS OF C-JUN MESSENGER-RNA AND JUN PROTEIN-LIKE IMMUNOREACTIVITY IN MOTOR AND SENSORY NEURONS FOLLOWING AXON DAMAGE
LONG-TERM INCREASE IN THE LEVELS OF C-JUN MESSENGER-RNA AND JUN PROTEIN-LIKE IMMUNOREACTIVITY IN MOTOR AND SENSORY NEURONS FOLLOWING AXON DAMAGE
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DOI：
10.1016/0304-3940(91)90731-8
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发表时间：
1991-08-05
影响因子：
2.5
通讯作者：
HUNT, SP
中科院分区：
文献类型：
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作者：
JENKINS, R;HUNT, SP
直接早期基因c-fos、c-jun和NGFI-A在有害感觉刺激后在浅表背角神经元中快速、短暂表达。然而，使用原位杂交来绘制mRNA或特异性抗体来检测蛋白质产物，我们无法在受刺激的背根神经节细胞或运动神经元中检测到这些基因表达的任何变化。相反，坐骨神经切片或挤压后，背根神经节细胞和运动神经元中c-jun mRNA和蛋白样免疫反应性（但不包括c-fos或NGFI-A）水平大量增加。然而，这些变化既不是迅速的，也不是短暂的。基因产物在神经损伤后24小时增加，但2小时没有增加，这些水平维持到7天后。这些结果表明，神经系统中c-jun基因表达的调控有多种途径，c-jun可能在神经元对损伤的反应中起关键作用。
The immediate early genes c-fos, c-jun and NGFI-A are rapidly and transiently expressed in neurons of the superficial dorsal horn following noxious sensory stimulation. However, using either in situ hybridisation to map mRNA or specific antibodies to detect the protein products we were unable to detect any change in expression of those genes in stimulated dorsal root ganglion cells or motor neurons. In contrast levels of c-jun mRNA and protein-like immunoreactivity (but not c-fos or NGFI-A) are massively increased within dorsal root ganglion cells and motor neurons following sciatic nerve section or crush. However, these changes are neither rapid nor transient. Increased gene product is seen at 24 h but not 2 h after nerve damage and these levels are maintained up to seven days later. These results suggest that there are multiple routes for the control of c-jun gene expression within the nervous system and that c-jun may play a key role in the neuronal response to injury.