Relationship Between GABAergic Interneurons Migration and Early Neocortical Network Activity
Relationship Between GABAergic Interneurons Migration and Early Neocortical Network Activity
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DOI：
10.1002/dneu.20696
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Voigt, Thomas
中科院分区：
文献类型：
--
作者：
de Lima, Ana D.;Gieseler, Anne;Voigt, Thomas
现有证据表明，在大脑皮层的早期发育过程中，自发网络活动的出现与发育中的皮层细胞迁移期的结束在时间上重叠。本研究在新皮层神经网络培养模型中探讨了神经元迁移的功能调控，采用时间间隔法检测迁移运动，钙离子成像法评价迁移神经元的活性，抗免疫细胞化学法回顾性鉴定迁移细胞。在细胞培养中，早期生理发育和细胞迁移在局部网络水平上再现，从而允许独立于地形复杂性的细胞迁移和网络发育之间的相互关系的研究。神经元在体外迁移至少12天，与非GABA能神经元相比，GABA能神经元迁移更快。迁移活动的下降与自发同步网络活动的发展是一致的。迁移的中间神经元不参与同步网络活动，但在观察时间内结束细胞迁移的中间神经元经常在不到一个小时的时间内参与同步活动。GABA（A）和离子型谷氨酸受体拮抗剂的应用显着增加了迁移的GABA能神经元的数量，而不改变迁移运动的动力学。因此，早期网络活动释放的神经递质可能有利于神经元迁移的终止。这些结果加强了网络活动在晚生GABA能细胞的发育中起重要作用的观点。(C)2008 Wiley Periodicals，Inc.开发神经生物学69：105-123，2009
Available evidence converges to suggest that during the early development of the cerebral cortex, the emergence of the spontaneous network activity chronologically overlap with the end of the cell migration period in the developing cortex. We approached the functional regulation of neuronal migration in a culture model of neocortical networks, using time lapses to detect migratory movements, calcium-imaging to assess the activity of migratory neurons, anti immunocytochemical methods to identify the migratory cells retrospectively. In cell cultures, early physiological development and cell migration are reproduced at a local network level, thus allowing the study of the interrelationships between cell migration and network development independent of the topographical complexity. Neurons migrate at least until 12 days in vitro and GABAergic neurons migrate faster compared with non-GABAergic neurons. A decline of migratory activity was coincident with the development of spontaneous synchronous network activity. Migrating interneurons did not participate in synchronous network activity, but interneurons that ended cell migration during observation time frequently engaged in synchronous activity within less than an hour. Application of GABA(A) and ionotropic glutamate receptor antagonists significantly increased the number of migrating GABAergic neurons without changing the dynamics of the migratory movements. Thus, neurotransmitters released by early network activity might favor the termination of neuronal migration. These results reinforce the idea that network activity plays an important role in the development of late-born GABAergic cells. (C) 2008 Wiley Periodicals, Inc. Develop Neurobiol 69: 105-123, 2009