CROSSED IMMUNOELECTROPHORESIS AS APPLIED TO STUDIES ON COMPLEX-FORMATION - BINDING OF HEPARIN TO ANTITHROMBIN-III AND ANTITHROMBIN-III-THROMBIN COMPLEX
CROSSED IMMUNOELECTROPHORESIS AS APPLIED TO STUDIES ON COMPLEX-FORMATION - BINDING OF HEPARIN TO ANTITHROMBIN-III AND ANTITHROMBIN-III-THROMBIN COMPLEX
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DOI：
10.1016/0022-1759(77)90138-7
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发表时间：
1977-01-01
影响因子：
2.2
通讯作者：
HENNINGSSON, E
中科院分区：
文献类型：
--
作者：
ANDERSSON, LO;ENGMAN, L;HENNINGSSON, E
用二维免疫电泳法获得了肝素与纯化的抗凝血酶III[兔]和抗凝血酶III-凝血酶复合体结合的信息。含有肝素的凝胶中各组分的流动性差异使抗凝血酶III的游离型和复合型得以区分。用纯化制剂获得的结果表明，肝素与抗凝血酶III的结合比与抗凝血酶III-凝血酶复合体的结合更强。在血浆中，肝素与几种成分结合，只有一小部分与抗凝血酶III结合。在血清中可以检测到几种含有抗凝血酶III的成分。
A 2-dimensional immunoelectrophoretic method was used to obtain information on the binding of heparin to purified antithrombin III [rabbit] and the antithrombin III-thrombin complex. The difference in mobility of the components in a gel containing heparin enables distinction between free and complexed forms of antithrombin III. The results obtained with purified preparations show that heparin is bound more strongly to antithrombin III than to the antithrombin III-thrombin complex. In plasma, heparin is bound to several components, only a fraction being bound to antithrombin III. Several components containing antithrombin III are detectable in serum [human].