IMMUNOLOGICAL MEMORY TO HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-B AND GLYCOPROTEIN-D IN MICE
IMMUNOLOGICAL MEMORY TO HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-B AND GLYCOPROTEIN-D IN MICE
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DOI：
10.1099/0022-1317-71-4-863
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发表时间：
1990-04-01
影响因子：
3.8
通讯作者：
NASH, AA
中科院分区：
文献类型：
--
作者：
BLACKLAWS, BA;NASH, AA
用组成表达1型单纯疱疹病毒糖蛋白B或D（分别为LTKgB和LTKgD）的小鼠L细胞系来研究小鼠对这些病毒糖蛋白的免疫应答的寿命。需要用细胞系进行两次免疫才能诱导持续抗体反应（存在超过200天）。只有LTKgD在小鼠中诱导了中和抗体反应，并且在两次接种后200天内仍保持高滴度。免疫后270天，小鼠体内也存在L细胞中表达的gB特异性前体细胞毒性T淋巴细胞。用这些细胞系免疫的小鼠耳廓的病毒清除率增加，接种LTKgD在免疫后7天的清除率高于LTKgB。这种类型的保护随着接种后时间的推移而降低，直到第161天，免疫组和对照组之间的病毒滴度没有显著差异。然而，LTKgD免疫接种可以防止小鼠神经节潜伏感染的建立，甚至在接种后186天。
Mouse L cell lines constitutively expressing glycoproteins B or D of herpes simplex virus type 1 (LTKgB and LTKgD respectively) were used to study the longevity of the immune response to these viral glycoproteins in mice. Two immunizations with the cell lines were necessary to induce a persisting antibody response (present for over 200 days). Only LTKgD induced a neutralizing antibody response in mice and this also remained at high titres over 200 days after two inoculations. The presence in mice of precursor cytotoxic T lymphocytes specific for gB expressed in the L cells was also shown up to 270 days after immunization. Mice immunized with the cell lines showed an increased rate of virus clearance from the ear pinna, inoculation with LTKgD resulting in more clearance than LTKgB at 7 days post-immunization. This type of protection was reduced with time after inoculation, until by day 161 there was no significant difference in virus titres between immunized and control groups. However, LTKgD immunization protected against the establishment of latent infections in the ganglia of mice even up to 186 days postinoculation.