SELECTIVE INDUCTION OF BRAIN TYPE-II NA+ CHANNELS BY NERVE GROWTH-FACTOR
SELECTIVE INDUCTION OF BRAIN TYPE-II NA+ CHANNELS BY NERVE GROWTH-FACTOR
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DOI：
10.1073/pnas.85.3.924
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发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
BREHM, P
中科院分区：
文献类型：
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作者：
MANDEL, G;COOPERMAN, SS;BREHM, P
源自大鼠嗜铬细胞瘤（PC12 细胞）的细胞只有在用神经生长因子处理后才能产生动作电位。使用电生理学方法，我们发现神经生长因子处理的 PC12 细胞中动作电位的出现可以通过 Na+ 通道密度的增加来解释。 PC12 细胞中 Na+ 通道的功能特性与周围神经描述的类似，但似乎与注射了脑 II 型 Na+ 通道 mRNA 的非洲爪蟾卵母细胞中合成的 Na+ 通道不同。为了确定 PC12 细胞是否表达大脑 II 型 Na+ 通道基因，我们使用可以区分大脑 I 型和 II 型 Na+ 通道 mRNA 的探针进行了 RNase 保护分析。这些研究的结果表明，未分化的PC12细胞表达II型Na+通道基因，但不表达I型Na+通道基因。神经生长因子治疗可增加 II 型 Na+ 通道基因的表达，但对 I 型基因表达没有影响。我们的研究结果表明，PC12 细胞中 Na+ 通道的兴奋性是由于神经生长因子对脑 II 型基因的特异性诱导所致。
Cells derived from a rat pheochromocytoma (PC12 cells) can generate an action potential only upon treatment with nerve growth factor. Using electrophysiological methods, we found that the appearance of action potentials in nerve growth factor-treated PC12 cells can be explained by an increase in the density of Na+ channels. The functional properties of Na+ channels in PC12 cells are similar to those described for peripheral nerves but appear to be different from Na+ channels synthesized in Xenopus oocytes injected with brain type II Na+-channel mRNA. To determine if PC12 cells express the brain type II Na+-channel gene, we performed RNase-protection analyses using probes that can distinguish between the brain type I and type II Na+-channel mRNAs. The results from these studies indicate that undifferentiated PC12 cells express the type II but not the type I Na+-channel gene. Treatment with nerve growth factor increases expression of the type II Na+-channel gene but has no effect on type I gene expression. Our findings suggest that Na+-channel excitability in PC12 cells is due to the specific induction of the brain type II gene by nerve growth factor.