Three-dimensional ultrastructure analysis of organelles in injured motor neuron.
Three-dimensional ultrastructure analysis of organelles in injured motor neuron.
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DOI：
10.1007/s12565-023-00720-y
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发表时间：
2023-07
影响因子：
1.2
通讯作者：
Tamada, Hiromi
中科院分区：
文献类型：
--
作者：
Tamada, Hiromi
细胞器的形态学分析是了解细胞状态和细胞发生机制的重要线索之一。特别是，在拥挤的细胞内细胞器的组织内的纳米级信息提供了更直接的影响时，相比于在培养或隔离的细胞的分析。然而，在使用光学显微镜（包括超分辨率显微镜）检测个体形状时存在一些困难。透射电子显微镜（TEM），其中超微结构可以在膜水平成像，不能确定整个结构，并对其进行定量分析。体积EM，如聚焦离子束/扫描电子显微镜（FIB/SEM），可以是一个强大的工具，探索三维超微结构的细节，甚至在一定的体积，并从他们测量几个参数。在这篇综述中，FIB/SEM分析在细胞器研究中的优势被强调沿着的线粒体分析在损伤的运动神经元的介绍。这将有助于了解线粒体的形态细节，特别是那些分布在细胞体以及轴突起始段（AIS）在小鼠组织中。这些地区还没有被探索到目前为止，由于在访问他们的图像条件显微镜遇到的困难。本文还结合实验结果讨论了神经再生的一些机制。最后，对FIB/SEM的发展前景进行了展望。生物化学和遗传学的细胞器结构的理解和纳米级的三维分布和形态的理解相结合，将有助于匹配在基因组学和结构生物学的成就。在线版本包含补充材料，可通过10.1007/s12565-023-00720-y获得。
Morphological analysis of organelles is one of the important clues for understanding the cellular conditions and mechanisms occurring in cells. In particular, nanoscale information within crowded intracellular organelles of tissues provide more direct implications when compared to analyses of cells in culture or isolation. However, there are some difficulties in detecting individual shape using light microscopy, including super-resolution microscopy. Transmission electron microscopy (TEM), wherein the ultrastructure can be imaged at the membrane level, cannot determine the whole structure, and analyze it quantitatively. Volume EM, such as focused ion beam/scanning electron microscopy (FIB/SEM), can be a powerful tool to explore the details of three-dimensional ultrastructures even within a certain volume, and to measure several parameters from them. In this review, the advantages of FIB/SEM analysis in organelle studies are highlighted along with the introduction of mitochondrial analysis in injured motor neurons. This would aid in understanding the morphological details of mitochondria, especially those distributed in the cell bodies as well as in the axon initial segment (AIS) in mouse tissues. These regions have not been explored thus far due to the difficulties encountered in accessing their images by conditional microscopies. Some mechanisms of nerve regeneration have also been discussed with reference to the obtained findings. Finally, future perspectives on FIB/SEM are introduced. The combination of biochemical and genetic understanding of organelle structures and a nanoscale understanding of their three-dimensional distribution and morphology will help to match achievements in genomics and structural biology. The online version contains supplementary material available at 10.1007/s12565-023-00720-y.
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DOI：
10.1083/jcb.202208005
发表时间：
2023-02-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
通讯作者：
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影响因子：
6.2
作者：
Clark, Kareem C.;Josephson, Anna;Dupree, Jeffrey L.
通讯作者：
Dupree, Jeffrey L.
影响因子：
9
作者：
Area-Gomez E;de Groof A;Bonilla E;Montesinos J;Tanji K;Boldogh I;Pon L;Schon EA
通讯作者：
Schon EA
影响因子：
2.9
作者：
Maclachlan C;Sahlender DA;Hayashi S;Molnár Z;Knott G
通讯作者：
Knott G
影响因子：
16
作者：
Csordás G;Várnai P;Golenár T;Roy S;Purkins G;Schneider TG;Balla T;Hajnóczky G
通讯作者：
Hajnóczky G