Effects of Lewis Y antigen on the gene expression of multiple drug resistance-associated proteins in human ovarian cancer RMG-I-H cells
Effects of Lewis Y antigen on the gene expression of multiple drug resistance-associated proteins in human ovarian cancer RMG-I-H cells
复制标题
DOI：
10.1007/s12032-009-9317-6
复制
发表时间：
2010-09-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Shulan
中科院分区：
文献类型：
--
作者：
Gao, Song;Liu, Qing;Zhang, Shulan
Lewis Y抗原对人卵巢癌RMG-I-H细胞中多种耐药相关蛋白基因表达的影响目前尚不清楚。在本研究中，我们检测了RMG-I-H细胞和抗Lewis Y单克隆抗体处理的RMG-I-H细胞中多种耐药相关蛋白（MRP）的基因表达，以探讨Lewis Y抗原与耐药相关蛋白基因表达之间的关联。与RMG-I细胞相比，RMG-I-H细胞中MRP1、MRP2、蛋白激酶C-α（PKC-α）和拓扑异构酶I（Topo I）mRNA的表达显着上调，而MDR-1 mRNA表达下调。免疫化学分析表明，RMG-I-H细胞中MDR-1蛋白（P-gp）的体外和体内表达水平显着高于RMG-I细胞。 RMG-I-H细胞用抗Lewis Y单克隆抗体处理后，随着处理时间的延长，MDR-1、MRP I、MRP2、PKC-α和Topo I mRNA的表达水平逐渐降低。相比之下，非治疗组中这些 mRNA 的表达水平没有明显变化。治疗后6 h，抗体治疗组MDR-1、MRP1、MRP2、PKC-α和Topo I mRNA的相对水平显着低于非治疗组。总之，Lewis Y抗原与调节多种耐药相关蛋白的基因表达密切相关。
The effects of Lewis Y antigen on the gene expression of multiple drug resistance-associated proteins in human ovarian cancer RMG-I-H cells were unclear by now. In this study, we detected the gene expression of multiple drug resistance-associated proteins (MRP) in RMG-I-H cells and RMG-I-H cells treated with anti-Lewis Y monoclonal antibody to investigate the association between Lewis Y antigen and the gene expression of drug resistance-associated proteins. Compared with RMG-I cells, the expression of MRP1, MRP2, protein kinase C-alpha (PKC-alpha), and topoisomerase I (Topo I) mRNAs in RMG-I-H cells were significantly upregulated, while the MDR-1 mRNA was downregulated. Immunochemistry analyses indicated that the in vitro and in vivo expression levels of MDR-1 protein (P-gp) in RMG-I-H cells were significantly higher than those in RMG-I cells. After RMG-I-H cells were treated with anti-Lewis Y monoclonal antibody, the expression levels of MDR-1, MRP I, MRP2, PKC-alpha, and Topo I mRNAs gradually decreased with the prolongation of treatment duration. In contrast, no obvious changes were noted in the expression levels of these mRNAs in the non-treatment group. At 6 h after treatment, the relative levels of MDR-1, MRP1, MRP2, PKC-alpha, and Topo I mRNAs in the antibody treatment group were significantly lower than those in the non-treatment group. In conclusion, Lewis Y antigen is closely associated with regulating the gene expression of multiple drug resistance-associated proteins.