Disease-specific protein corona sensor arrays may have disease detection capacity
Disease-specific protein corona sensor arrays may have disease detection capacity
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DOI：
10.1039/c9nh00097f
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发表时间：
2019-09-01
影响因子：
9.7
通讯作者：
Mahmoudi, Morteza
中科院分区：
文献类型：
--
作者：
Caracciolo, Giulio;Safavi-Sohi, Reihaneh;Mahmoudi, Morteza
早期的任何灾难性疾病（E。例如，在一个实施例中，癌症）被诊断出来，它就越有可能被治疗，从而提供改善的患者预后、延长的生存期和更好的生活质量。在2014年初，我们发现各种类型的疾病可以显著影响蛋白质冠的组成/概况（即，在纳米颗粒与生物流体相互作用时在纳米颗粒表面形成的生物分子层）。在这里，通过结合疾病特异性蛋白质冠和传感器阵列技术的概念，我们开发了一个具有使用血浆进行疾病检测能力的平台。我们的传感器阵列由三个交叉反应的脂质体，具有不同的脂质成分和表面电荷。传感器阵列提供吸附在脂质体上的冠蛋白组合物的模式识别，而不是检测特定的生物标志物。作为可行性研究，使用从诊断为五种不同癌症类型的患者获得的血浆样品进行传感器阵列验证（即，e.肺癌、胶质母细胞瘤、脑膜瘤、骨髓瘤和胰腺癌）和健康供体的对照组。虽然没有单一的冠组成是特定的任何一种癌症类型，重叠，但不同的模式的冠组成构成了一个独特的“指纹”为每一种类型的癌症（具有高的分类准确性，即。e. 99.4%）。为了最终探索这种传感器阵列早期检测癌症的能力，我们使用了从健康人中获得的队列血浆，这些人在血浆采集后几年被诊断为肺癌、脑癌和胰腺癌。我们的研究结果表明，疾病特异性蛋白质冠传感器阵列不仅有助于疾病的筛选，检测和鉴定，而且还可能有助于识别新的蛋白质模式标记，其在疾病发展和/或疾病生物学中的作用迄今尚未得到重视。
The earlier any catastrophic disease (e. g., cancer) is diagnosed, the more likely it can be treated, providing improved patient prognosis, extended survival and better quality of life. In early 2014, we revealed that various types of disease can substantially affect the composition/ profile of protein corona (i.e., a layer of biomolecules that forms at the surface of nanoparticles upon their interactions with biological fluids). Here, by combining the concepts of disease-specific protein corona and sensor array technology we developed a platform with disease detection capacity using blood plasma. Our sensor array consists of three cross-reactive liposomes, with distinct lipid composition and surface charge. Rather than detecting a specific biomarker, the sensor array provides pattern recognition of the corona protein composition adsorbed on the liposomes. As a feasibility study, sensor array validation was performed using plasma samples obtained from patients diagnosed with five different cancer types (i. e. lung cancer, glioblastoma, meningioma, myeloma, and pancreatic cancer) and a control group of healthy donors. Although no single corona composition is specific for any one cancer type, overlapping but distinct patterns of the corona composition constitutes a unique `` fingerprint'' for each type of cancer (with a high classification accuracy, i. e. 99.4%). To finally probe the capacity of this sensor array for early detection of cancers, we used cohort plasma obtained from healthy people who were subsequently diagnosed several years after plasma collection with lung, brain, and pancreatic cancers. Our results suggest that the disease-specific protein corona sensor array will not only be instrumental in the screening, detection, and identification of diseases, but may also help identify novel protein pattern markers whose role in disease development and/ or disease biology has not been appreciated so far.