Prevalence and Viral Loads of Cutaneous Human Polyomaviruses in the Skin of Patients With Chronic Inflammatory Skin Diseases
Prevalence and Viral Loads of Cutaneous Human Polyomaviruses in the Skin of Patients With Chronic Inflammatory Skin Diseases
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DOI：
10.1093/infdis/jiy618
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发表时间：
2019-05-15
影响因子：
6.4
通讯作者：
Daibata, Masanori
中科院分区：
文献类型：
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作者：
Hashida, Yumiko;Higuchi, Tomonori;Daibata, Masanori
背景人类皮肤微生物与各种皮肤疾病有关。然而，大多数研究都集中在细菌群落，而对通常驻留的皮肤病毒如多瘤病毒科及其与皮肤疾病的关联知之甚少。我们使用从86名日本炎症性皮肤病和蕈样肉芽肿患者的皮损和非皮损皮肤采集的三联皮肤拭子以及149名健康对照个体，调查了默克尔细胞多瘤病毒（MCPyV）、人多瘤病毒6型（HPyV 6）和人多瘤病毒7型（HPyV 7）的感染水平。这项年龄匹配的病例对照研究首次分析了与各种皮肤病相关的多瘤病毒载量。在银屑病患者中，HPyV 6/HPyV 7的病毒载量显著较高，而MCPyV的病毒载量较低。在大多数患者中，不仅在病变部位观察到病毒载量变化，而且在临床未受影响的皮肤部位也观察到病毒载量变化。检测的病毒株均为亚洲/日本基因型。我们的研究结果表明，在某些炎症性皮肤疾病的皮肤多瘤病毒感染水平的协变。为了了解这些改变对炎症性皮肤病发病机制的影响，有必要进行全球前瞻性纵向研究。
Background. Human skin microorganisms have been associated with various skin diseases. However, most studies have focused on bacterial communities, and little is known about normally resident skin viruses such as the Polyomaviridae and their association with cutaneous disorders.Methods. We investigated the infection levels of Merkel cell polyomavirus (MCPyV), human polyomavirus 6 (HPyV6), and human polyomavirus 7 (HPyV7), using triplet skin swabs collected from lesional and nonlesional skins of 86 Japanese patients with inflammatory skin diseases and mycosis fungoides, and from 149 healthy control individuals.Results. This age-matched case-control study provides the first analyses of the loads of polyomaviruses in association with various skin diseases. The viral loads were significantly higher for HPyV6/HPyV7 and lower for MCPyV in patients with psoriasis. The viral load variation was observed not only at lesion sites, but also at clinically unaffected skin sites in most of the patients. The viral strains tested were all of the Asian/Japanese genotype.Conclusions. Our findings suggest a covariation in the infection levels of cutaneous polyomaviruses in certain inflammatory skin conditions. Worldwide prospective longitudinal studies are warranted to understand the influence of such alterations on the pathogenesis of inflammatory skin disorders.