Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)
Cloning, mapping, and in vivo localization of a human member of the PKCI-1 protein family (PRKCNH1)
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DOI：
10.1006/geno.1996.0435
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发表时间：
1996-08-15
期刊：
影响因子：
4.4
通讯作者：
Christman, MF
中科院分区：
文献类型：
--
作者：
Brzoska, PM;Chen, HY;Christman, MF
我们在这里报告完整的cDNA序列，基因组图谱，和免疫定位的第一个人类成员的蛋白激酶C抑制剂（PKCI-1）基因家族。预测的人蛋白（hPKCI-1）与牛的同源性为96%，与玉米的同源性为53%，表明该蛋白家族具有很高的进化保守性。通过荧光原位杂交将hPKCI-1基因（HGV批准的符号PRKCNH 1）定位于人类染色体5q31.2。间接免疫荧光显示，hPKCI-1定位于人成纤维细胞系的细胞质中的细胞骨架结构，并且在很大程度上被排除在细胞核之外。hPKCI-1的细胞质定位与介导电离辐射应答的膜源性信号的假定作用一致。(C)出版社：Academic Press，Inc.
We report here the complete cDNA sequence, genomic mapping, and immunolocalization of the first human member of the protein kinase C inhibitor (PKCI-1) gene family. The predicted human protein (hPKCI-1) is 96% identical to bovine and 53% identical to maize members, indicating the great evolutionary conservation of this protein family. The hPKCI-1 gene (HGMV-approved symbol PRKCNH1) maps to human chromosome 5q31.2 by fluorescence in situ hybridization. Indirect immunofluorescence shows that hPKCI-1 localizes to cytoskeletal structures in the cytoplasm of a human fibroblast cell line and is largely excluded from the nucleus. The cytoplasmic localization of hPKCI-1 is consistent with a postulated role in mediating a membrane-derived signal in response to ionizing radiation. (C) 1996 Academic Press, Inc.