Impact of lifestyle on cytochrome P450 monooxygenase repertoire is clearly evident in the bacterial phylum Firmicutes
Impact of lifestyle on cytochrome P450 monooxygenase repertoire is clearly evident in the bacterial phylum Firmicutes
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DOI：
10.1038/s41598-020-70686-8
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发表时间：
2020-08-19
影响因子：
4.6
通讯作者：
Syed, Khajamohiddin
中科院分区：
文献类型：
--
作者：
Padayachee, Tiara;Nzuza, Nomfundo;Syed, Khajamohiddin
细胞色素P450单加氧酶（CYP/P450）是一种血红素巯基化蛋白，在生物体的初级代谢和次级代谢中发挥重要作用。对动物、植物、卵菌和真菌等真核生物的研究表明，这些生物体中的P450谱受其生活方式的影响。然而，生活方式对细菌中P450谱的影响很少报道。这项研究就是这样一个例子，生活方式的影响似乎深刻地影响了属于厚壁菌门的细菌物种中的P450谱。对属于158属的972种厚壁菌门物种的P450进行全基因组分析显示，只有属于37属的229种具有P450; 38%的芽孢杆菌属物种，其次是14%的梭菌属和2.7%的其他厚壁菌门物种具有P450。致病性或嗜酸性生活方式影响P450含量的程度使得属于链球菌属、李斯特菌属、葡萄球菌属、乳杆菌属、乳球菌属和明串珠菌属的物种不具有P450，除了少数葡萄球菌属物种具有单个P450。只有18%的P450被发现参与次生代谢，并预测有89个P450在特定的次生代谢产物的合成中起作用。本研究是首次对厚壁菌门P450进行综合分析的报道。
Cytochrome P450 monooxygenases (CYPs/P450s), heme thiolate proteins, are well known for their role in organisms' primary and secondary metabolism. Research on eukaryotes such as animals, plants, oomycetes and fungi has shown that P450s profiles in these organisms are affected by their lifestyle. However, the impact of lifestyle on P450 profiling in bacteria is scarcely reported. This study is such an example where the impact of lifestyle seems to profoundly affect the P450 profiles in the bacterial species belonging to the phylum Firmicutes. Genome-wide analysis of P450s in 972 Firmicutes species belonging to 158 genera revealed that only 229 species belonging to 37 genera have P450s; 38% of Bacilli species, followed by 14% of Clostridia and 2.7% of other Firmicutes species, have P450s. The pathogenic or commensal lifestyle influences P450 content to such an extent that species belonging to the genera Streptococcus, Listeria, Staphylococcus, Lactobacillus, Lactococcus and Leuconostoc do not have P450s, with the exception of a handful of Staphylococcus species that have a single P450. Only 18% of P450s are found to be involved in secondary metabolism and 89 P450s that function in the synthesis of specific secondary metabolites are predicted. This study is the first report on comprehensive analysis of P450s in Firmicutes.