Flavonoid transport across RBE4 cells: A blood-brain barrier model.
Flavonoid transport across RBE4 cells: A blood-brain barrier model.
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DOI：
10.2478/s11658-010-0006-4
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发表时间：
2010-06
影响因子：
8.3
通讯作者：
Calhau C
中科院分区：
文献类型：
--
作者：
Faria A;Pestana D;Teixeira D;Azevedo J;De Freitas V;Mateus N;Calhau C
人们对饮食治疗策略越来越感兴趣，以对抗氧化应激诱导的中枢神经系统（CNS）损伤，中枢神经系统损伤与许多病理生理过程相关，包括阿尔茨海默病和帕金森病以及脑血管疾病。由于缺乏关于这些化合物进入CNS能力的信息，识别与酚类神经保护相关的机制被推迟。本研究的目的是评估黄酮类化合物跨RBE-4细胞（大鼠脑毛细血管内皮细胞的永生化细胞系）的跨膜转运和乙醇对这种转运的影响。使用HPLC-DAD（二极管阵列检测器）对研究样品（基底外侧介质）中的所有酚类化合物进行检测和定量。所有测试的类黄酮（儿茶素、槲皮素和矢车菊素-3-葡萄糖苷）以时间依赖性方式穿过RBE-4细胞。该转运不受0.1%乙醇存在的影响。总之，所测试的黄酮类化合物能够穿过该血脑屏障模型。
There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.