Actin-based motility of endosomes is linked to the polar tip growth of root hairs.
Actin-based motility of endosomes is linked to the polar tip growth of root hairs.
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DOI：
10.1016/j.ejcb.2004.12.029
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发表时间：
2005-07
影响因子：
6.6
通讯作者：
Boris Voigt;A. Timmers;J. Šamaj;A. Hlavacka;T. Ueda;M. Preuss;E. Nielsen;J. Mathur;N. Emans;H. Stenmark;A. Nakano;F. Baluška;D. Menzel
中科院分区：
文献类型：
--
作者：
Boris Voigt;A. Timmers;J. Šamaj;A. Hlavacka;T. Ueda;M. Preuss;E. Nielsen;J. Mathur;N. Emans;H. Stenmark;A. Nakano;F. Baluška;D. Menzel
植物尖端生长被认为是一个以肌动蛋白为基础的细胞过程，需要在生长锥发生靶向胞吐和代偿性内吞。然而，在植物中，参与极化膜运输途径的亚细胞区室的身份仍然是谜。在这里，我们描述了尖端生长的根毛细胞的内体室。我们证明了它们在生长尖端的存在和尖端生长停止后的差异分布。我们还表明，核内体的存在以及它们在尖端区域内的快速运动取决于完整的肌动蛋白细胞骨架，并涉及肌动蛋白聚合。综上所述，肌动蛋白推动的内体运动与根毛极尖生长密切相关。
Plant tip growth has been recognized as an actin-based cellular process requiring targeted exocytosis and compensatory endocytosis to occur at the growth cone. However, the identity of subcellular compartments involved in polarized membrane trafficking pathways remains enigmatic in plants. Here we characterize endosomal compartments in tip-growing root hair cells. We demonstrate their presence at the growing tip and differential distribution upon cessation of tip growth. We also show that both the presence of endosomes as well as their rapid movements within the tip region depends on an intact actin cytoskeleton and involves actin polymerization. In conclusion, actin-propelled endosomal motility is tightly linked to the polar tip growth of root hairs.