Serological characterization of anti-endotoxin serum directed against the conjugate of oligosaccharide core of Escherichia coli type R4 with tetanus toxoid
Serological characterization of anti-endotoxin serum directed against the conjugate of oligosaccharide core of Escherichia coli type R4 with tetanus toxoid
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DOI：
10.1016/s0928-8244(03)00104-4
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发表时间：
2003-06-10
影响因子：
--
通讯作者：
Lugowski, C
中科院分区：
文献类型：
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作者：
Lukasiewicz, J;Jachymek, W;Lugowski, C
采用还原性胺化反应制备了大肠杆菌R4型低聚糖核与破伤风类毒素的共价偶联物。新糖缀合物是兔体内良好的免疫原，能产生高水平的IgG类抗脂多糖（LPS）抗体。发现抗血清能够与相同（R4）或相关（RI）核心型的光滑LPS分子发生反应。酶联免疫吸附试验和免疫印迹试验显示了这些反应。流式细胞术显示，抗核心抗体与LPS反应，存在于完整的，活的，光滑的细菌标记的细胞超过90%。体外抗os R4-TT血清显示出较高的内毒素中和活性。血清抑制内毒素诱导的J-774A.1细胞合成肿瘤坏死因子a和一氧化氮，减轻YAC-1细胞的肺潴留。(C) 2003欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
The covalent conjugate of oligosaccharide core of Escherichia coli type R4 with tetanus toxoid was prepared using reaction of reductive amination. The neoglycoconjugate was a good immunogen in rabbits yielding a high level of anti-lipopolysaccharide (LPS) antibodies of the IgG class. It was found that antiserum was able to react with the smooth LPS molecules of identical (R4) or related (RI) core type. The reactions were shown in the enzyme-linked immunosorbent assay and the immunoblotting test. Flow cytometry showed that anti-core antibodies reacted with LPS present on intact, live, smooth bacteria labelling more than 90% of cells. The anti-OS R4-TT serum used for in vitro studies showed high endotoxin neutralization activity. The serum inhibited endotoxin-induced tumor necrosis factor a and nitric oxide synthesis by the J-774A.1 cell line and attenuated pulmonary retention of YAC-1 cells. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.