Analysis of the transcriptional complexity of Arabidopsis thaliana by massively parallel signature sequencing
Analysis of the transcriptional complexity of Arabidopsis thaliana by massively parallel signature sequencing
复制标题
DOI：
10.1038/nbt992
复制
发表时间：
2004-08-01
影响因子：
46.9
通讯作者：
Haudenschild, CD
中科院分区：
文献类型：
--
作者：
Meyers, BC;Vu, TH;Haudenschild, CD
对短 mRNA 衍生标签进行大规模测序可以建立复杂转录组的定性和定量特征。我们使用大规模并行特征测序 (MPSS) 对拟南芥五个不同文库中的 12,304,362 个标签进行了测序。总共 48,572 个不同的签名，每个代表不同的转录本，均以显着水平表达。将这些特征与拟南芥基因组序列的注释进行比较；在五个文库中，该比较产生了 17,353 至 18,361 个具有正义表达的基因，以及 5,487 至 8,729 个具有反义表达的基因。另外 6,691 个 MPSS 签名映射到基因组的未注释区域。 1,168 个基因的表达得到了证实，而这些基因的表达数据以前是未知的。在这些文库中转录的拟南芥基因中，观察到了超过 25% 的替代多腺苷酸化。 MPSS 表达数据表明拟南芥转录组很复杂，并且包含许多正常编码转录本的尚未表征的变体。
Large-scale sequencing of short mRNA-derived tags can establish the qualitative and quantitative characteristics of a complex transcriptome. We sequenced 12,304,362 tags from five diverse libraries of Arabidopsis thaliana using massively parallel signature sequencing (MPSS). A total of 48,572 distinct signatures, each representing a different transcript, were expressed at significant levels. These signatures were compared to the annotation of the A. thaliana genomic sequence; in the five libraries, this comparison yielded between 17,353 and 18,361 genes with sense expression, and between 5,487 and 8,729 genes with antisense expression. An additional 6,691 MPSS signatures mapped to unannotated regions of the genome. Expression was demonstrated for 1,168 genes for which expression data were previously unknown. Alternative polyadenylation was observed for more than 25% of A. thaliana genes transcribed in these libraries. The MPSS expression data suggest that the A. thaliana transcriptome is complex and contains many as-yet uncharacterized variants of normal coding transcripts.