Development of motor innervation of the chick following dorsal-ventral limb bud rotations
Development of motor innervation of the chick following dorsal-ventral limb bud rotations
复制标题
背腹肢芽旋转后雏鸡运动神经支配的发育
DOI：
10.1523/jneurosci.03-09-01760.1983
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
B. Ferguson
中科院分区：
文献类型：
--
作者：
B. Ferguson
为了测试运动神经元可以用来生长到合适的靶点的机制，我在运动神经元生长之前绕着背腹轴旋转了肢体。然后用逆行辣根过氧化物酶摄取法确定支配单个肌肉和肌群的脊髓运动神经元的位置。在背侧-腹侧肢体旋转后，在运动神经元死亡前后，运动神经元对其相应的肌肉进行神经支配。因此，细胞死亡并不能消除肢体背腹旋转后运动核团与相应肌肉之间匹配的错误。运动神经元在生长之前必须为外周靶点指定，它们相对直接地生长到该靶点。轴突首先聚集在适合正常肢体方向的位置，然后在神经丛和近端神经干内移动背腹位置，以补偿肢体旋转。根据这些结果，推测支配背侧或腹侧肌肉的轴突在生长过程中可能使用化学特异性线索，以保持神经内相对于肢体方向的适当位置。
To test mechanisms which motoneurons may use to grow to their appropriate targets, I rotated the limb around the dorsal-ventral axis prior to motoneuron outgrowth. The positions of motoneurons in the spinal cord innervating individual muscles and muscle masses were then determined using retrograde horseradish peroxidase uptake. Motoneurons innervated their appropriate muscles after dorsal-ventral limb rotation, before and after motoneuron death. Thus, cell death does not serve to remove errors in matching between motor nuclei and their corresponding muscles after dorsoventral rotation of the limb. Motoneurons must be specified for a peripheral target prior to outgrowth, and they grow to that target relatively directly. Axons compensated for the limb rotation by first collecting into groups in a position appropriate for the normal limb orientation, then shifting dorsal-ventral position within the plexus and proximal nerve trunk. Based on these results it is hypothesized that axons destined to innervate dorsal or ventral musculature might use chemospecific cues during growth to maintain appropriate positions within the nerve with respect to limb orientation.