Regulation of CLV3 expression by two homeobox genes in Arabidopsis
Regulation of CLV3 expression by two homeobox genes in Arabidopsis
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DOI：
10.1104/pp.001867
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发表时间：
2002-06-01
期刊：
影响因子：
7.4
通讯作者：
Simon, R
中科院分区：
文献类型：
--
作者：
Brand, U;Grünewald, M;Simon, R
分生组织连续产生新器官的能力取决于其干细胞群体的活性，这些器官位于分生组织尖端。在拟南芥中，干细胞结构域的大小受两种拮抗活性调节。编码同源域蛋白的WUS（Wuschel）基因促进了干细胞的形成和维持。这些干细胞表达CLV3（Clavata3），以及通过CLV1/CLV2受体复合物的CLV3信号限制了WUS活性。可以通过反馈调节来实现干细胞种群的稳态，从而使干细胞数的变化导致CLV3表达水平的相应变化，并通过CLV信号转导途径调整WUS表达。我们已经分析了CLV3的表达是由WUS的活性或其他同源基因的STM（芽分生生物）的活性控制的，这是干细胞维持所需的。我们发现CLV3的表达仅取决于WUS功能，仅在胚芽分生组织中。在后来的发育阶段，WUS与STM一起促进了CLV3表达的水平。在分生组织中，通过表达CLV3来应对WUS活动的能力仅限于分生组织。
The ability of meristems to continuously produce new organs depends on the activity of their stem cell populations, which are located at the meristem tip. In Arabidopsis, the size of the stem cell domain is regulated by two antagonistic activities. The WUS (WUSCHEL) gene, encoding a homeodomain protein, promotes the formation and maintenance of stem cells. These stem cells express CLV3 (CLAVATA3), and signaling of CLV3 through the CLV1/CLV2 receptor complex restricts WUS activity. Homeostasis of the stem cell population may be achieved through feedback regulation, whereby changes in stem cell number result in corresponding changes in CLV3 expression levels, and adjustment of WUS expression via the CLV signal transduction pathway. We have analyzed whether expression of CLV3 is controlled by the activity of WUS or another homeobox gene, STM (SHOOT MERISTEMLESS), which is required for stem cell maintenance. We found that expression of CLV3 depends on WUS function only in the embryonic shoot meristem. At later developmental stages, WUS promotes the level of CLV3 expression, together with STM. Within a meristem, competence to respond to WUS activity by expressing CLV3 is restricted to the meristem apex.