LOCALIZATION OF STRUCTURAL PROTEINS IN AFRICAN SWINE FEVER VIRUS-PARTICLES BY IMMUNOELECTRON MICROSCOPY
LOCALIZATION OF STRUCTURAL PROTEINS IN AFRICAN SWINE FEVER VIRUS-PARTICLES BY IMMUNOELECTRON MICROSCOPY
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DOI：
10.1128/jvi.58.2.377-384.1986
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发表时间：
1986-05-01
影响因子：
5.4
通讯作者：
VINUELA, E
中科院分区：
文献类型：
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作者：
CARRASCOSA, JL;GONZALEZ, P;VINUELA, E
用免疫电镜技术对7种非洲猪瘟病毒结构蛋白进行了定位。在包埋和薄切片之前或之后，将非洲猪瘟病毒感染的细胞与对不同结构蛋白具有特异性的单克隆抗体孵育，并在用蛋白A-金复合物标记后，在电子显微镜下检查样品。蛋白质p14和p24被发现在病毒粒子的外部区域，蛋白质p12，p72，p17和p37被发现在中间层，和蛋白质p150被发现在类核和一个顶点。识别p150蛋白和p220（一种病毒诱导的非结构蛋白）的单克隆抗体也可以与未感染和病毒感染细胞核中的一种成分结合。
Seven African swine fever virus structural proteins were localized in the virion by immunoelectron microscopy. African swine fever virus-infected cells were incubated, before or after embedding and thin sectioning, with monoclonal antibodies specific for different structural proteins, and after labelling with protein A-gold complexes, the samples were examined in the electron microscope. Proteins p14 and p24 were found in the external region of the virion, proteins p12, p72, p17, and p37 were found in the intermediate layers, and protein p150 was found in the nucleoid and in one vertex. A monoclonal antibody that recognized protein p150 as well as p220, a virus-induced, nonstructural protein, could also bind to a component present in the nucleus of both uninfected and virus-infected cells.