Protective effect of palmitoylethanolamide in a rat model of cystitis.
Protective effect of palmitoylethanolamide in a rat model of cystitis.
复制标题
棕榈酰乙醇酰胺对大鼠膀胱炎模型的保护作用。
DOI：
10.1016/j.juro.2014.11.083
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Izzo
中科院分区：
文献类型：
--
作者：
F. Pessina;R. Capasso;F. Borrelli;T. Aveta;Lorena Buono;G. Valacchi;P. Fiorenzani;V. Di Marzo;P. Orlando;A. Izzo
目的PEA是一种与内源性大麻素一起从膜磷脂中释放出来的内源性介质。它是一种植物来源的化合物，具有止痛和抗炎特性。我们验证了实验性膀胱炎的病理生理机制是否涉及前列腺素A及其部分靶标，即CB1和CB2受体，以及PPARα水平的变化。我们还确定了外源性给予PEA是否可以作为膀胱炎的预防措施。材料和方法用环磷酰胺诱导雌性大鼠膀胱炎。记录伤害性反应、排尿次数、大体损害、髓过氧化物酶活性、膀胱重量、膀胱PEA和内源性大麻素水平(用液相色谱-质谱仪测定)和PEA靶标的表达(用定量逆转录聚合酶链式反应测定)。结果环磷酰胺诱导的疼痛行为、膀胱炎症和排尿功能障碍与膀胱PEA水平升高、CB1受体表达上调、PPARα表达下调、CB2受体表达无变化有关。外源性给药，超微粉PEA减轻疼痛行为，排空和膀胱肉眼损害。CB1拮抗剂利莫那班和PPARα拮抗剂GW6471可抵消PEA对大体损伤的有利作用。此外，GW6471还进一步减少了PEA治疗大鼠的排尿次数。结论本研究提供了强有力的证据，证明PEA在环磷酰胺诱导的膀胱炎中具有保护作用，并改变了PEA及其一些靶点的膀胱水平。
PurposePEA is an endogenous mediator released together with the endocannabinoid anandamide from membrane phospholipids. It is a plant derived compound with analgesic and anti-inflammatory properties. We verified whether the pathophysiology of experimental cystitis involves changes in the levels of PEA and of some of its targets, ie CB1and CB2receptors, and PPARα. We also determined whether exogenously administered PEA could be proposed as a preventive measure for cystitis.Materials and MethodsCystitis was induced by cyclophosphamide in female rats. Nociceptive responses, voiding episodes, gross damage, myeloperoxidase activity, bladder weight, bladder PEA and endocannabinoid levels (measured by liquid chromatography-mass spectrometry) and the expression of PEA targets (measured by quantitative reverse transcriptase-polymerase chain reaction) were recorded.ResultsCyclophosphamide induced pain behavior, bladder inflammation and voiding dysfunction associated with increased bladder levels of PEA, up-regulation of CB1receptor mRNA expression, down-regulation of PPARα mRNA and no change in CB2receptor mRNA expression. Exogenously administered, ultramicronized PEA attenuated pain behavior, voids and bladder gross damage. The CB1antagonist rimonabant and the PPARα antagonist GW6471 counteracted the beneficial effect of PEA on gross damage. Also, GW6471 further decreased voiding episodes in rats treated with PEA.ConclusionsThe current study provides strong evidence for a protective role of PEA as well as an alteration in bladder levels of PEA and of some of its targets in cyclophosphamide induced cystitis.
影响因子：
6.6
作者：
Wang, Zun-Yi;Wang, Peiqing;Bjorling, Dale E.
通讯作者：
Bjorling, Dale E.
影响因子：
4.5
作者：
Fuellhase, Claudius;Campeau, Lysanne;Andersson, Karl-Erik
通讯作者：
Andersson, Karl-Erik