TOPOGRAPHY OF THE 3 LATE MESSENGER-RNAS OF POLYOMA-VIRUS WHICH ENCODE THE VIRION PROTEINS
TOPOGRAPHY OF THE 3 LATE MESSENGER-RNAS OF POLYOMA-VIRUS WHICH ENCODE THE VIRION PROTEINS
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DOI：
10.1128/jvi.33.2.637-651.1980
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
PARKER, J
中科院分区：
文献类型：
--
作者：
KAMEN, R;FAVALORO, J;PARKER, J
利用抗核酸酶s1 RNA-DNA杂交体的1维和2维凝胶电泳技术，将分别编码多瘤病毒3种衣壳蛋白（VP1、VP2和VP3）的3个胞质聚腺苷化mRNA定位到病毒基因组上。mRNA，被命名为mVP1， mVP2和mVP3，表明从RNA的共沉淀和信使活性推断出的编码功能，包括一组重叠的3''-共末端分子，它们也共享一个异构的非编码5''-末端区域家族。3种植物的共线编码区长度不同，该共线编码区与5”先导序列拼接在一起。在图单元25.3处常见的聚腺苷化3”端图。mVP1、mVP3和mVP2共线体的5”端分别位于48.5、59.5和66.5个图单元。对多瘤病毒DNA序列在剪接位点附近进行检查，这些剪接位点由已知中间序列末端共有的序列S1凝胶作图数据所近似，从而可以预测多瘤病毒晚期mRNA的精确剪接点。在所有三种情况下，先导序列在每个特定信使所使用的翻译起始密码子之前至少48个核苷酸连接到mRNA体上。在每个mRNA中起作用的起始信号是剪接后的第一个AUG（或GUG）三重态。
The 3 cytoplasmic polyadenylated mRNA which separately encode the 3 capsid proteins (VP1, VP2 and VP3) of polyoma virus were mapped on the viral genome by 1- and 2-dimensional gel electrophoresis of nuclease S1-resistant RNA-DNA hybrids. The mRNA, designated mVP1, mVP2 and mVP3 to indicate the coding functions deduced from the cosedimentation of the RNA and the messenger activities comprise an overlapping set of 3''-coterminal molecules which also share a heterogeneous family of noncoding 5''-terminal regions. The 3 species differ in the length of the 3'' colinear coding region which is spliced to the 5'' leader sequences. The common polyadenylated 3'' end maps at map unit 25.3. The 5'' ends of the colinear bodies of mVP1, mVP3 and mVP2 map at 48.5, 59.5 and 66.5 map units, respectively. An examination of the polyoma virus DNA sequence in the vicinities of splicing sites approximated by the S1 gel mapping data for sequences common to the ends of known intervening sequences allowed prediction of the precise splice points in polyoma virus late mRNA. In all 3 cases, the leader sequences are joined to the mRNA bodies at least 48 nucleotides before the translational initiation codon used in each particular messenger. The start signal which functions in each mRNA is the 1st AUG (or GUG) triplet after the splice junction.