Submergence stress-induced hypocotyl elongation through ethylene signaling-mediated regulation of cortical microtubules in Arabidopsis.
Submergence stress-induced hypocotyl elongation through ethylene signaling-mediated regulation of cortical microtubules in Arabidopsis.
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DOI：
10.1093/jxb/erz453
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发表时间：
2019-10
影响因子：
6.9
通讯作者：
Xiaohong Wang;Q. Ma;Ran Wang;Pan Wang;Yimin Liu;Tonglin Mao
中科院分区：
文献类型：
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作者：
Xiaohong Wang;Q. Ma;Ran Wang;Pan Wang;Yimin Liu;Tonglin Mao
植物生长响应于淹水胁迫而显著改变。然而，在陆生植物如拟南芥中，幼苗响应这种胁迫，特别是下胚轴生长的分子机制在很大程度上还不清楚。微管细胞骨架参与植物细胞的生长，但目前还不清楚是否淹没介导的植物生长涉及微管细胞骨架。在本研究中，我们表明，淹没诱导水下下胚轴伸长通过激活乙烯信号，调节皮层微管重组拟南芥。浸水增强乙烯信号，然后激活和稳定其下游转录因子，光敏色素相互作用因子3（PIF3），促进下胚轴伸长。特别是，微管组织的调节对于这一生理过程非常重要。微管不稳定蛋白60（MDP60），这是以前确定的PIF3的下游效应，发挥了积极的作用，在浸水诱导下胚轴伸长。淹水通过乙烯信号转导诱导MDP60表达。在mdp60突变体中，淹水对下胚轴伸长和皮层微管重组的影响被抑制。这些数据表明，一个潜在的机制，其中淹没激活乙烯信号，促进水下下胚轴伸长通过改变微管细胞骨架在拟南芥。
Plant growth is significantly altered in response to submergence stress. However, the molecular mechanisms used by the seedlings in response to this stress, especially for hypocotyl growth, are largely unclear in terrestrial plants, such as Arabidopsis thaliana. The microtubule cytoskeleton participates in plant cell growth, but it remains unclear whether submergence-mediated plant growth involves the microtubule cytoskeleton. In the present study, we demonstrated that submergence induced underwater hypocotyl elongation through the activation of ethylene signaling, which modulates cortical microtubule reorganization in Arabidopsis thaliana. Submergence enhanced ethylene signaling, which then activated and stabilized its downstream transcription factor, phytochrome-interacting factor 3 (PIF3), to promote hypocotyl elongation. In particular, the regulation of microtubule organization was important for this physiological process. The microtubule-destabilizing protein 60 (MDP60), which was previously identified as a downstream effector of PIF3, played a positive role in submergence-induced hypocotyl elongation. Submergence induced MDP60 expression through ethylene signaling. The effects of submergence on hypocotyl elongation and cortical microtubule reorganization were suppressed in the mdp60 mutants. These data suggest a potential mechanism in which submergence activates ethylene signaling to promote underwater hypocotyl elongation via the alteration of the microtubule cytoskeleton in Arabidopsis.