INVITRO METHYLATION AND DEMETHYLATION OF METHYL-ACCEPTING CHEMOTAXIS PROTEINS IN BACILLUS-SUBTILIS
INVITRO METHYLATION AND DEMETHYLATION OF METHYL-ACCEPTING CHEMOTAXIS PROTEINS IN BACILLUS-SUBTILIS
复制标题
DOI：
10.1021/bi00307a010
复制
发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
ORDAL, GW
中科院分区：
文献类型：
--
作者：
GOLDMAN, DJ;ORDAL, GW
枯草芽孢杆菌对引诱剂的反应是通过去甲基化一组完整的膜蛋白，即甲基接受趋化蛋白（MCP）。在由膜囊泡和纯化的甲基转移酶和甲基酯酶组成的体外系统中研究了这些蛋白的甲基化和去甲基化。化学引诱剂天冬氨酸抑制MCP的甲基化并刺激其去甲基化。在枯草芽孢杆菌酶存在的情况下，大肠杆菌放射性标记膜对天冬氨酸没有反应，去甲基化率增加。枯草芽孢杆菌MCP被多次甲基化。去甲基化导致十二烷基硫酸钠-聚丙烯酰胺凝胶上的蛋白质迁移速度减慢。
B. subtilis responds to attractants by demethylating a group of integral membrane proteins referred to as methyl-accepting chemotaxis proteins (MCP). The methylation and demethylation of these proteins were studied in an in vitro system, consisting of membrane vesicles and purified methyltransferase and methylesterase. The chemoattractant aspartate inhibited methylation and stimulated demethylation of MCP. Escherichia coli radiolabeled membranes in the presence of B. subtilis enzyme did not respond to aspartate by an increased demethylation rate. B. subtilis MCP were multiply methylated. Demethylation resulted in slower migrating proteins on sodium dodecyl sulfate-polyacrylamide gels.