Live imaging of Arabidopsis development.
Live imaging of Arabidopsis development.
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DOI：
10.1007/978-1-62703-580-4_28
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发表时间：
2014
影响因子：
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通讯作者：
Daniel von Wangenheim;Gabor Daum;J. Lohmann;Ernst H. K. Stelzer;A. Maizel
中科院分区：
文献类型：
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作者：
Daniel von Wangenheim;Gabor Daum;J. Lohmann;Ernst H. K. Stelzer;A. Maizel
活细胞成像是研究活植物在正常或胁迫生长条件下细胞结构、动力学和功能的基本方法。拟南芥非常适合于各种活显微镜技术。在这一章中，我们提供了设计实时成像实验的指导原则。我们具体讨论了每种显微技术各自的优点、报告材料的选择和样品的制备。提供了用于地上部和根部成像的详细方案。
Live cell imaging is an essential methodology for studying the structure, dynamics, and functions of cells in a living plant under normal or stressed growth conditions.Arabidopsis thalianais perfectly amenable to various live microscopy techniques. In this chapter, we provide guidelines to design live-imaging experiments. We discuss specifically the respective advantage of each microscopy technique, the choice of reporter, and the preparation of the sample. Detailed protocols for imaging of shoot and roots are provided.